(→Migration of plasmids containing gBlocks digestion) |
(→Visualization) |
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Result of the migration | Result of the migration | ||
+ | File:T--Paris_Saclay--160701_visualization_gBlocks2_échelle.jpg#file | ||
For strains containing plasmids with [[Team:Paris_Saclay/Notebook/June/28#pPS16_007|pPS16_007]], according to the digestion products, 2 clones had the plasmid with the good insert. Indeed we could observe on the gel the two expected fragments of 2.7kb and 0.7kb). All the same for strains containing gBlocks 4.2 and GFP 1-9, 3 and 2 clones were good respectively. However, DNA concentration was too low. | For strains containing plasmids with [[Team:Paris_Saclay/Notebook/June/28#pPS16_007|pPS16_007]], according to the digestion products, 2 clones had the plasmid with the good insert. Indeed we could observe on the gel the two expected fragments of 2.7kb and 0.7kb). All the same for strains containing gBlocks 4.2 and GFP 1-9, 3 and 2 clones were good respectively. However, DNA concentration was too low. | ||
For others strains, migration products did not get consistent results, probably due to errors during the extraction. | For others strains, migration products did not get consistent results, probably due to errors during the extraction. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 11:34, 19 July 2016