Difference between revisions of "Team:Paris Saclay/Notebook/July/1"
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Digestion products from 30/06/16 were migrated on a gel (0.8% agarose) for 30 min at 100 V. | Digestion products from 30/06/16 were migrated on a gel (0.8% agarose) for 30 min at 100 V. | ||
| + | [[File:T--Paris_Saclay--160701_visualization_gBlocks2_échelle.jpg#file|200px|thumb|left|4.1, 4.2 and GFP 1-9 Migration]] | ||
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For strains containing plasmids with [[Team:Paris_Saclay/Notebook/June/28#pPS16_007|pPS16_007]], according to the digestion products, 2 clones had the plasmid with the good insert. Indeed we could observe on the gel the two expected fragments of 2.7kb and 0.7kb). All the same for strains containing gBlocks 4.2 and GFP 1-9, 3 and 2 clones were good respectively. However, DNA concentration was too low. | For strains containing plasmids with [[Team:Paris_Saclay/Notebook/June/28#pPS16_007|pPS16_007]], according to the digestion products, 2 clones had the plasmid with the good insert. Indeed we could observe on the gel the two expected fragments of 2.7kb and 0.7kb). All the same for strains containing gBlocks 4.2 and GFP 1-9, 3 and 2 clones were good respectively. However, DNA concentration was too low. | ||
For others strains, migration products did not get consistent results, probably due to errors during the extraction. | For others strains, migration products did not get consistent results, probably due to errors during the extraction. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 11:37, 19 July 2016
