Difference between revisions of "Team:USP UNIFESP-Brazil/Integrated Practices"

Latest revision as of 05:45, 20 November 2016

AlgAranha Team USP_UNIFESP-Brazil

iGEM 2016

Integrated Practices

Our integrated practices are sprawled trought our entire project, we kindly ask to check this links:

Introduction of Human Practices

Integrated Human Practices

Lab equipment in Brazil

Hardware

When we think about an iGEM project, we have to wonder: what are the people’s necessities? How can we contribute to the world? Are we able to develop a new technology? What difficulties will we have to face?

We had to answer all those questions, and also face all the obstacles that could prevent the completion of our project. Brazil is not a developed country, and it presents a direct impacts on science. It is common to hear complaints about the difficulty to buy a laboratory device. Sometimes, even a simple equipment, as a centrifuge is not available, and as a manner to solve this issue, we explored the open hardware framework.

Open hardware consists in sharing information about hardware designs, offering many benefits to their users. The main advantage of its use is the possibility of building a new technology in a cheaper and faster manner. Besides, as the source code and all the instructions to build the devices are available, it is also easier to update, maintain and repair it.

The iGEM group and the open hardware community collaboration enabled the development of a centrifuge, an electrophoresis system and a transilluminator. All of these devices were essentials to the project and now we are excited to keep this collaboration. The continuous use of the equipments also increases chances of something going wrong, like in one of our electrophoresis runs when a power supply blowed up and, we got in critical situation. The repairment is very expensive and would take a long time to get it done. And there is no code available to just check which part went wrong.

More than only setting up a great hardware kit, we also interacted intensively with a broad audience. We engaged the USP community directly with a well structured Hackathon, got in touch directly with teachers and high school students at the FabLearn conference and spread the Open Lab Hardware word even for designers and artists at FAZ!

BioHackathon at USP

In the hope of reaching out to the community and effectively making an impact with our work, we organized a hands-on open workshop on making open hardware equipment for biotech. The event was organized in partnership with University of Sao Paulo (USP) Synthetic Biology Club, USP Free Hardware group, USP entrepreneurship nucleus and the agrotech company Lotan, founded by former iGEMers. This partnership made the event possible and the company even adopted some hardware made, the Seletora Centrifuge, the Transilluminator Tabajara and one Electrophoresis Chamber. The rest of the equipment produced in the event was either given to the participants at purchase cost or was directly used by our team to develop our project. More than reaching out to the entrepreneurship community and former iGEMers, we had also a participant from USP-EEL-Brazil 2016 iGEM team, which travelled 200km just for the event!

The microcentrifuge and the other equipment we’ve built costed a great deal less than a commercial one, but the most important part is the autonomy and freedom that having these Open Hardware pieces promotes. The experience on making these equipments will surely guarantee that this culture will continue, not only among our team members, but among all the students that have attended our workshop. These students with different knowledge backgrounds, from biology to humanities to engineering, also are helping to build a community, wih insights that are making the Open Hardwares more functional, practical and accessible!
Fablearn

The brazilian edition of the FabLearn Conference was held by the University of São Paulo (USP) and the Stanford University during the 9th and 10th of September, 2016, at the campus of USP. We presented Seletora Centrifuge and Electrophoresis System using digital fabrication techniques. The conference gave us the opportunity to share experiences in research and education based in the “learning by doing” principle. We talked, for example high school students who built a syringe pump using Arduino and were planning to build a centrifuge as well in a really insightful meeting. We perceived a great interest from teachers, professors, high school and undergrad students who also encouraged us to provide a repository with our designs and projects so they could reproduce the devices themselves. This acceptance reveals that the practical impact of our project goes beyond research, transforming education and learning.

FAZ

The FAZ ("make" verb in impetartive form, portuguese) festival was held at the Red Bull Station, in Sao Paulo downtown, and gathered a very diverse group of people interested in the maker culture. Our team presented the hardware built using digital fabrication techniques in a lecture and demonstration session. The event represented a singular opportunity to share our work and insights: from the motivations that led us to built our own lab equipments to the context of the iGEM competition and the social impact of our project. As we were used to talking to scholars and students, FAZ gave us the chance to reach out for designers, architects and artists, a public we had not dialogued before. The insights and questions raised there enriched our experience by pointing out the design and visual aspects of building biotechnology devices. It was great to see how different backgrounds lead to different ways of approaching an specific problem.

Accordingly to our principles of openess, we provided manuals, for constructing your own centrifuge, transilluminator and photodocumentation and Electrophoresis Chamber with a step by step image guide in order to help anyone build its own laboratory equipments. This guide is in our Hardware Wiki page

Accordingly to our principles of openess, we provide here a manual, with a step by step image guide in order to help you build your own centrifuge for your next iGEM project!
Click here to download the manual to build your own Seletora Centrifuge

Zip files

Even though we were not able to produce such a detailed material for all our systems, here we provided the files for the 3D printing and laser cutting needed to produce them at your home town (or any place with a FabLab, at least)!

Here you can download the Electrophoresis System files,

And here you can download the Transilluminator Tabajara files
Finally, here you can download the files related to 3D printing, laser cutting and the Arduino code for making your own Seletora Centrifuge!

You can click here to give a look at the Hardware page!

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 				<h2> Integrated Practices</h2>
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-Check this links:
+Our integrated practices are sprawled trought our entire project, we kindly ask to check this links:
 <p><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/HP">Introduction of Human Practices</a></p>
@@ Line 108: / Line 106: @@
-<p class=black>Along with the iGEM project’s development we dealt with a limited access to centrifuges, electrophoresis systems and transilluminators, all essential equipments to molecular biology techniques. It was very difficult to plan in advance our experiments due to the restricted access to some laboratories, since most equipments at USP are responsibility of different professors and researchers. Thus, building our own hardware using a laser cutter, electronic components and 3D printer had a great impact in the execution of our project. Our <i>do-it-yourself</i> centrifuge gave us independence and autonomy to perform the routine experiments, like a simple DNA extraction, gel purification or PCR purification as well, all indispensable steps for basic cloning. </p>
+<p class="black">
+When we think about an iGEM project, we have to wonder: what are the people’s necessities? How can we contribute to the world? Are we able to develop a new technology? What difficulties will we have to face?</p>
-<p class=black>The continuous use of the equipments also increases chances of something going wrong, like in one of our electrophoresis runs when a power supply burned out and we got in critical situation since its repairment is very expensive and would take a long time to get it done. Once again, we were compelled to build our own version of a lab device, which was achieved by the cooperation with the Hardware Livre, a group about open hardware at USP. Other equipments are being developed like a transilluminator and an electrophoresis system. We also created the device named Cyclops to count cells on stream using a webcam adapted to function as a microscope with an increasing objective of 10 microns. It is possible to count cells considering their color and shape too, showing the versatility of this tool.</p>
+<p class="black">
+We had to answer all those questions, and also face all the obstacles that could prevent the completion of our project. Brazil is not a developed country, and it presents a direct impacts on science. It is common to hear complaints about the difficulty to buy a laboratory device. Sometimes, even a simple equipment, as a centrifuge is not available, and as a manner to solve this issue, we explored the open hardware framework.</p>
-<p class=black>Then we decided to organize an event that we could interact with other people, teaching them how to make a centrifuge and they could also suggest improvements to the next versions. Our event had support of a company called Lotan and a group dedicated to entrepreneurship at Universidade de São Paulo, named NEU. The purpose of the event was to promote knowledge exchange and it was achieved. People of different areas were present and contributed a lot with their opinions! </p>
-<p class=black>After this interesting experience, we went to other events about digital fabrication like the FAZ festival which was held at the Red Bull Station in São Paulo and gathered a very diverse group of people interested in the maker culture. Our team presented the hardware using digital fabrication techniques in a lecture and demonstration session. The event represented a singular opportunity to share our experience, from the motivations that led us to built our own lab equipments to the context of the iGEM competition and the social impact of our project. FAZ gave us the chance to reach out for designers, architects and artists, a public we had not impacted before. Their insights and questions enriched our experiences, it was great to know how different backgrounds lead to different ways of approaching the same thing. </p>
+<p class="black">
+Open hardware consists in sharing information about hardware designs, offering many benefits to their users. The main advantage of its use is the possibility of building a new technology in a cheaper and faster manner. Besides, as the source code and all the instructions to build the devices are available, it is also easier to update, maintain and repair it.</p>
-<p class=black>In order to impact the academic and educational communities our team submitted the project of the low cost centrifuge and electrophoresis system to the Brazilian edition of the FabLearn Conference, held by USP and the Stanford University at the campus of USP. There is much in common with the iGEM principles, including the protagonism and empowering of researchers and students. We talked to some high school students who built a syringe pump using Arduino and they were planning to build a centrifuge as well. This situation was a two-way path: their tips will certainly save us a lot of time when we were building a pump while our experience in building a centrifuge will certainly be useful to them. We perceived a great interest from those who came to see our project, including teachers, professors, high school and undergrad students who also encouraged us to provide a repository with our designs and projects so they could reproduce the devices themselves. This acceptance reveals that the practical impact of our project goes beyond research, transforming education and learning.</p>
-<p class=black>By all these experiences with open hardware, inside and outside the lab, we could see how essential it was for the project as a whole. Moreover, we generated awareness about our contribution to the progress of science and education.</p>
+<p class="black">
+The iGEM group and the open hardware community collaboration enabled the development of a centrifuge, an electrophoresis system and a transilluminator.  All of these devices were essentials to the project and now  we are excited to keep this collaboration. The continuous use of the equipments also increases chances of something going wrong, like in one of our electrophoresis runs when a power supply blowed up and, we got in critical situation.
+The repairment is very expensive and would take a long time to get it done. And there is no code available to just check which part went wrong. </p>
+<p class=black> More than only setting up a great hardware kit, we also interacted intensively with a broad audience. We engaged the USP community directly with a well structured Hackathon, got in touch directly with teachers and high school students at the FabLearn conference and spread the Open Lab Hardware word even for designers and artists at FAZ! </p>
+<p class=black><strong>BioHackathon at USP</p>
+<p class=black>In the hope of reaching out to the community and effectively making an impact with our work, we organized a hands-on open workshop on making open hardware equipment for biotech. The event was organized in partnership with University of Sao Paulo (USP) Synthetic Biology Club, USP Free Hardware group, USP entrepreneurship nucleus and the agrotech company Lotan, founded by former iGEMers. This partnership made the event possible and the company even adopted some hardware made, the Seletora Centrifuge, the Transilluminator Tabajara and one Electrophoresis Chamber. The rest of the equipment produced in the event was either given to the participants at  purchase cost or  was directly used by our team to develop our project. More than reaching out to the entrepreneurship community and former iGEMers, we had also a participant from USP-EEL-Brazil 2016 iGEM team, which travelled 200km just for the event! </p>
+<p class=black>The microcentrifuge and the other equipment we’ve built costed a great deal less than a commercial one, but the most important part is the autonomy and freedom that having these Open Hardware pieces promotes. The experience on making these equipments will surely guarantee that this culture will continue, not only among our team members, but among all the students that have attended our workshop. These students with different knowledge backgrounds, from biology to humanities to engineering, also are helping to build a community, wih insights that are making the Open Hardwares more functional, practical and accessible! </>
+<p class=black><strong>Fablearn</p>
+<p class=black>	The brazilian edition of the FabLearn Conference was held by the University of São Paulo (USP) and the Stanford University during the 9th and 10th of September, 2016, at the campus of USP. We presented Seletora Centrifuge and Electrophoresis System using digital fabrication techniques. The conference gave us the opportunity to share experiences in research and education based in the “learning by doing” principle. We talked, for example high school students who built a syringe pump using Arduino and were planning to build a centrifuge as well in a really insightful meeting.
+We perceived a great interest from teachers, professors, high school and undergrad students who also encouraged us to provide a repository with our designs and projects so they could reproduce the devices themselves. This acceptance reveals that the practical impact of our project goes beyond research, transforming education and learning.</p>
+<p class=black><strong>FAZ</p>
+<p class=black>The FAZ ("make" verb in impetartive form, portuguese) festival was held at the Red Bull Station, in Sao Paulo downtown, and gathered a very diverse group of people interested in the maker culture. Our team presented the hardware built using digital fabrication techniques in a lecture and demonstration session. The event represented a singular opportunity to share our work and insights: from the motivations that led us to built our own lab equipments to the context of the iGEM competition and the social impact of our project. As we were used to talking to scholars and students, FAZ gave us the chance to reach out for designers, architects and artists, a public we had not dialogued before. The insights and questions raised there enriched our experience by pointing out the design and visual aspects of building biotechnology devices. It was great to see how different backgrounds lead to different ways of approaching an specific problem.  </p>
+<p class=black>Accordingly to our principles of openess, we provided manuals, for constructing your own centrifuge,  transilluminator and photodocumentation and Electrophoresis Chamber
+with a step by step image guide in order to help anyone build its own laboratory equipments. This guide is in our <a target="_blank" href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Hardware">Hardware Wiki page</a></p>
+<p class=black>Accordingly to our principles of openess, we provide here a manual, with a step by step image guide in order to help you build your own centrifuge for your next iGEM project!</p>
+<a href=https://static.igem.org/mediawiki/2016/d/de/T--USP_UNIFESP-Brazil--Seletora_centrifuge_manual.pdf>Click here to download the manual to build your own Seletora Centrifuge<a></u>
+<div class="small-10 columns small-offset-2">
+<div class="small-10 small-offset-1 columns">
+<br></br>
+</div>
+</div>
+<center> <p class=black><strong> Zip files</p></strong></center>
+<p class=black> Even though we were not able to produce such a detailed material for all our systems, here we provided the files for the 3D printing and laser cutting needed to produce them at your home town (or any place with a FabLab, at least)! </p>
+<p class=black> Here you can download the <a href= https://static.igem.org/mediawiki/2016/1/13/T--USP_UNIFESP-Brazil--ElectrophoresisSystem.zip>Electrophoresis System</a> files, </p>
+<p class=black> And here you can download the <a href= https://static.igem.org/mediawiki/2016/6/65/T--USP_UNIFESP-Brazil--TransilluminatorTabajara.zip> Transilluminator Tabajara </a> files
+<p class=black> Finally, here you can download  the files related to 3D printing, laser cutting and the Arduino code for making your own <a href = https://static.igem.org/mediawiki/2016/9/96/T--USP_UNIFESP-Brazil--SeletoraCentrifugeFull.zip> Seletora Centrifuge</a>!</p>
+</div>
+</div>
+<div class="small-10 columns small-offset-2">
+<div class="small-10 small-offset-1 columns">
+<img style="max-width:130%!important;margin-left:-90px;"src="https://static.igem.org/mediawiki/2016/e/e4/T--USP_UNIFESP-Brazil--hackathon1.png">
+<img style="max-width:130%!important;margin-left:-90px;margin-top:20px;"src="https://static.igem.org/mediawiki/2016/d/d3/T--USP_UNIFESP-Brazil--hackathon2.png">
+<img src="https://static.igem.org/mediawiki/2016/e/e2/T--USP_UNIFESP-Brazil--FAZ.png">
 <p class=black><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Hardware">You can click here to give a look at the Hardware page!</a></p>
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 <img src="https://static.igem.org/mediawiki/2016/e/e2/T--USP_UNIFESP-Brazil--FAZ.png">
+</div>
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