Vivisiratuti (Talk | contribs) |
(AWards content transferred here) |
||
(31 intermediate revisions by 3 users not shown) | |||
Line 72: | Line 72: | ||
− | + | <!-- | |
<div class="row meio"> | <div class="row meio"> | ||
Line 85: | Line 85: | ||
<p>AlgAranha</p> | <p>AlgAranha</p> | ||
<div class="row"> | <div class="row"> | ||
+ | --> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
<a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Description"> | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Description"> | ||
− | <div class="small-3 columns membro" data-nome="PROJECT" data-img="https://static.igem.org/mediawiki/2016/ | + | <div class="small-3 columns membro" data-nome="PROJECT" data-img="https://static.igem.org/mediawiki/2016/5/59/T--USP_UNIFESP-Brazil--description.png"> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/ | + | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/5/59/T--USP_UNIFESP-Brazil--description.png')"> |
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black">DESCRIPTION</p> | + | <p class="black"><b>DESCRIPTION</b></p> |
</div> | </div> | ||
Line 98: | Line 104: | ||
<a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Experiments"> | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Experiments"> | ||
− | <div class="small-3 columns membro" data-nome="EXPERIMENTS" data-bio=" data-img="https://static.igem.org/mediawiki/2016/ | + | <div class="small-3 columns membro" data-nome="EXPERIMENTS" data-bio=" data-img="https://static.igem.org/mediawiki/2016/8/8a/T--USP_UNIFESP-Brazil--experiments.png"> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/ | + | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/8/8a/T--USP_UNIFESP-Brazil--experiments.png')"> |
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black">EXPERIMENTS</p> | + | <p class="black"><b>EXPERIMENTS</b></p> |
</div> | </div> | ||
<a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Proof"> | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Proof"> | ||
− | <div class="small-3 columns membro" data-nome="PROOF OF CONCEPT" data-img="https://static.igem.org/mediawiki/2016/b/ | + | <div class="small-3 columns membro" data-nome="PROOF OF CONCEPT" data-img="https://static.igem.org/mediawiki/2016/b/bb/T--USP_UNIFESP-Brazil--proof_of_concept.png"> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/b/ | + | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/b/bb/T--USP_UNIFESP-Brazil--proof_of_concept.png')"> |
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black">PROOF OF CONCEPT</p> | + | <p class="black"><b>PROOF OF CONCEPT</b></p> |
</div> | </div> | ||
− | <a href=" | + | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Results"> |
− | <div class="small-3 columns membro" data-nome="RESULTS" data-img="https://static.igem.org/mediawiki/2016/ | + | <div class="small-3 columns membro" data-nome="RESULTS" data-img="https://static.igem.org/mediawiki/2016/6/65/T--USP_UNIFESP-Brazil--results.png"> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/ | + | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/6/65/T--USP_UNIFESP-Brazil--results.png')"> |
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black">RESULTS</p> | + | <p class="black"><b>RESULTS</b></p> |
</div> | </div> | ||
− | <a href=" | + | </div> |
− | <div class="small-3 columns membro" data-nome="NOTEBOOK" data-img="https://static.igem.org/mediawiki/2016/ | + | <div class="small-12 small-centered columns"> <!--seg linha comeca aqui--> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/ | + | |
+ | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Notebook"> | ||
+ | <div class="small-3 columns small-offset-1 membro" data-nome="NOTEBOOK" data-img="https://static.igem.org/mediawiki/2016/6/62/T--USP_UNIFESP-Brazil--_notebook.png"> | ||
+ | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/6/62/T--USP_UNIFESP-Brazil--_notebook.png')"> | ||
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black">NOTEBOOK</p> | + | <p class="black"><b>NOTEBOOK</b></p> |
</div> | </div> | ||
− | <a href=" | + | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Safety"> |
− | <div class="small-3 columns membro" data-nome="SAFETY" data-img="https://static.igem.org/mediawiki/2016/0/ | + | <div class="small-3 columns membro" data-nome="SAFETY" data-img="https://static.igem.org/mediawiki/2016/0/0e/T--USP_UNIFESP-Brazil--safety_icon.png"> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/0/ | + | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/0/0e/T--USP_UNIFESP-Brazil--safety_icon.png')"> |
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black">SAFETY</p> | + | <p class="black"><b>SAFETY</b></p> |
</div> | </div> | ||
− | <a href=" | + | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Model"> |
− | <div class="small-3 columns membro" data-nome=" | + | <div class="small-3 columns membro" data-nome="MODELING" data-img="https://static.igem.org/mediawiki/2016/c/cb/T--USP_UNIFESP-Brazil--modelin.png"> |
− | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/ | + | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/c/cb/T--USP_UNIFESP-Brazil--modelin.png')"> |
<div class="overlay"> </div> | <div class="overlay"> </div> | ||
</a> | </a> | ||
</div> | </div> | ||
− | <p class="black"> | + | <p class="black"><b>MODELING</b></p> |
</div> | </div> | ||
+ | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Integrated_Practices"> | ||
+ | <div class="small-3 columns membro" data-nome="PROJECT" data-img="https://static.igem.org/mediawiki/2016/d/d3/T--USP_UNIFESP-Brazil--_integrated_practices.png"> | ||
+ | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/d/d3/T--USP_UNIFESP-Brazil--_integrated_practices.png')"> | ||
+ | <div class="overlay"> </div> | ||
+ | </a> | ||
+ | </div> | ||
+ | <p class="black"><b>INTEGRATED PRACTICES</b></p> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Hardware"> | ||
+ | |||
+ | <div class="small-3 columns membro" data-nome="EXPERIMENTS" data-bio=" data-img="https://static.igem.org/mediawiki/2016/2/27/T--USP_UNIFESP-Brazil--hardware.png"> | ||
+ | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/2/27/T--USP_UNIFESP-Brazil--hardware.png')"> | ||
+ | <div class="overlay"> </div> | ||
+ | </a> | ||
+ | |||
+ | </div> | ||
+ | <p class="black"><b>HARDWARE</b></p> | ||
+ | </div> | ||
+ | |||
+ | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Measurement"> | ||
+ | <div class="small-3 columns membro" data-nome="PROOF OF CONCEPT" data-img="https://static.igem.org/mediawiki/2016/8/8a/T--USP_UNIFESP-Brazil--measurement_.png"> | ||
+ | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/8/8a/T--USP_UNIFESP-Brazil--measurement_.png')"> | ||
+ | <div class="overlay"> </div> | ||
+ | </a> | ||
+ | </div> | ||
+ | <p class="black"><b>MEASUREMENT</b></p> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Plant"> | ||
+ | <div class="small-3 columns membro" data-nome="PROOF OF CONCEPT" data-img="https://static.igem.org/mediawiki/2016/9/9b/T--USP_UNIFESP-Brazil--_plant_synthetic_biology.png"> | ||
+ | <div class="foto-time" style="background-image: url('https://static.igem.org/mediawiki/2016/9/9b/T--USP_UNIFESP-Brazil--_plant_synthetic_biology.png')"> | ||
+ | <div class="overlay"> </div> | ||
+ | </a> | ||
+ | </div> | ||
+ | <p class="black"><b>PLANT SYNTHETIC BIOLOGY</b></p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
<a href="#top"> | <a href="#top"> | ||
+ | </div><!---segunda linha acaba aqui--> | ||
+ | <!-- | ||
<ul id=menu-lateral style="absolute; width:20%"> | <ul id=menu-lateral style="absolute; width:20%"> | ||
Line 177: | Line 235: | ||
</ul> | </ul> | ||
− | + | --> | |
<!-- | <!-- | ||
<div class="row meio"> | <div class="row meio"> | ||
Line 214: | Line 272: | ||
− | + | ||
</div> | </div> | ||
<br> | <br> | ||
Line 239: | Line 297: | ||
<p class="black">Our project was inspired by the UCLA’s projects presented at iGEM in 2014 and 2015 about synthetic silks. Like we said above, silks are very interesting material but one of the main problems is dealing with its high content of Cytosine and Guanine. Most of the expression systems are not able to deal with that, however we have known Chlamydomonas reinhardtii has naturally high CG content which makes it interesting as an expression chassis. One of UCLA’s projects was called Silk Functionalization: Developing the Next Generation of Performance Fibers. The team has created a genetic construct using the Green Fluorescent Protein (GFP) inserted between N- and C- terminal domains of Bombyx mori’s silk to express proteins able to bind to native silk proteins. When this customized GFPs and native proteins were mixed and spun they were able to recognize each other by the terminal domains, making a fluorescent silk.</p> | <p class="black">Our project was inspired by the UCLA’s projects presented at iGEM in 2014 and 2015 about synthetic silks. Like we said above, silks are very interesting material but one of the main problems is dealing with its high content of Cytosine and Guanine. Most of the expression systems are not able to deal with that, however we have known Chlamydomonas reinhardtii has naturally high CG content which makes it interesting as an expression chassis. One of UCLA’s projects was called Silk Functionalization: Developing the Next Generation of Performance Fibers. The team has created a genetic construct using the Green Fluorescent Protein (GFP) inserted between N- and C- terminal domains of Bombyx mori’s silk to express proteins able to bind to native silk proteins. When this customized GFPs and native proteins were mixed and spun they were able to recognize each other by the terminal domains, making a fluorescent silk.</p> | ||
− | |||
<p class="black">From this idea we thought: “Silk is a very interesting material and could be expressed in Chlamydomonas reinhardtii, a model organism that has many advantages and that remains underexplored. What could be put in place of a GFP to express in microalgae? What biomolecule could take advantage of this amazing silk feature? It has to work while immobilized... Why not enzymes? There are many studies about enzymes immobilization, enzymes are very appropriate and useful in many applications around the world!”. But we knew it could not to be any enzyme linked to silk, we knew it need to address technical, social and economic issues. So we read that spider silk has been studied to many biomedical applications, like the treatment of burn victims. Then we studied about burns in order to find an interesting enzyme that could solve some problem and we learned that one of the most important problems affecting burn victims is sepsis. In this way, we sought for antimicrobial enzymes and we found the endolysins. Endolysins are very interesting enzymes because they are able to combat multiresistant bacterias and they are specific, avoiding problems like the antibiotic resistance.</p> | <p class="black">From this idea we thought: “Silk is a very interesting material and could be expressed in Chlamydomonas reinhardtii, a model organism that has many advantages and that remains underexplored. What could be put in place of a GFP to express in microalgae? What biomolecule could take advantage of this amazing silk feature? It has to work while immobilized... Why not enzymes? There are many studies about enzymes immobilization, enzymes are very appropriate and useful in many applications around the world!”. But we knew it could not to be any enzyme linked to silk, we knew it need to address technical, social and economic issues. So we read that spider silk has been studied to many biomedical applications, like the treatment of burn victims. Then we studied about burns in order to find an interesting enzyme that could solve some problem and we learned that one of the most important problems affecting burn victims is sepsis. In this way, we sought for antimicrobial enzymes and we found the endolysins. Endolysins are very interesting enzymes because they are able to combat multiresistant bacterias and they are specific, avoiding problems like the antibiotic resistance.</p> | ||
Line 245: | Line 302: | ||
<p class="black">So we decided to make a project about spider silk production in microalgae, creating possibilities to future works with silk proteins from this amazing organism model, like the association between them and endolysins by UCLA’s approach in order to make an antimicrobial silk to be applied on burn victims to combat sepsis.</p> | <p class="black">So we decided to make a project about spider silk production in microalgae, creating possibilities to future works with silk proteins from this amazing organism model, like the association between them and endolysins by UCLA’s approach in order to make an antimicrobial silk to be applied on burn victims to combat sepsis.</p> | ||
+ | <p class=black><strong>References:</strong></p> | ||
+ | <p class="black">Eichler-Stahlberg, Alke et al. “Strategies to Facilitate Transgene Expression in Chlamydomonas Reinhardtii.” Planta 229.4 (2009): 873–883. Print.</p> | ||
+ | |||
+ | <p class="black">Fuhrmann, Markus et al. “A Synthetic Gene Coding for the Green Fluorescent Protein (GFP) Is a Versatile Reporter in Chlamydomonas Reinhardtii.” The Plant journal: for cell and molecular biology 19.3 (1999): 353–361. Print.</p> | ||
+ | |||
+ | <p class="black">Lewis, Randolph V. “Spider Silk: Ancient Ideas for New Biomaterials.” Chemical reviews 106.9 (2006): 3762–3774. Print.</p> | ||
+ | |||
+ | <p class="black">Lumbreras, Victoria et al. “Efficient Foreign Gene Expression in Chlamydomonas Reinhardtii Mediated by an Endogenous Intron.” The Plant journal: for cell and molecular biology 14.4 (1998): 441–447. Print.</p> | ||
+ | |||
+ | <p class="black">Mayfield, S. P., S. E. Franklin, and R. A. Lerner. “Expression and Assembly of a Fully Active Antibody in Algae.” Proceedings of the National Academy of Sciences 100.2 (2003): 438–442. Print.</p> | ||
+ | |||
+ | <p class="black">Mayfield, Stephen P. et al. “Chlamydomonas Reinhardtii Chloroplasts as Protein Factories.” Current opinion in biotechnology 18.2 (2007): 126–133. Print.</p> | ||
+ | |||
+ | <p class="black">Rasala, Beth A. et al. “Robust Expression and Secretion of Xylanase1 in Chlamydomonas Reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide.” PloS one 7.8 (2012): e43349. Print.</p> | ||
+ | |||
+ | <p class="black">Rosenberg, Julian N. et al. “A Green Light for Engineered Algae: Redirecting Metabolism to Fuel a Biotechnology Revolution.” Current opinion in biotechnology 19.5 (2008): 430–436. Print.</p> | ||
+ | |||
+ | <p class="black">Schroda, M., D. Blöcker, and C. F. Beck. “The HSP70A Promoter as a Tool for the Improved Expression of Transgenes in Chlamydomonas.” The Plant journal: for cell and molecular biology 21.2 (2000): 121–131. Print.</p> | ||
+ | |||
+ | <p class="black">Wijffels, René H., Olaf Kruse, and Klaas J. Hellingwerf. “Potential of Industrial Biotechnology with Cyanobacteria and Eukaryotic Microalgae.” Current opinion in biotechnology 24.3 (2013): 405–413. Print.</p> | ||
</div> | </div> | ||
Line 256: | Line 333: | ||
<div class="small-10 columns small-offset-2"> | <div class="small-10 columns small-offset-2"> | ||
<div class="small-10 small-offset-1 columns"> | <div class="small-10 small-offset-1 columns"> | ||
− | <p class="black"> | + | <p class="black">TEXTO AQUI</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<div class="small-10 columns small-offset-2 titulo-verde"> | <div class="small-10 columns small-offset-2 titulo-verde"> | ||
<div class="small-11 small-offset-1 columns"><a name="pro"></a> | <div class="small-11 small-offset-1 columns"><a name="pro"></a> | ||
Line 289: | Line 350: | ||
</div> | </div> | ||
</div> | </div> | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<div class="small-10 columns small-offset-2 titulo-verde"> | <div class="small-10 columns small-offset-2 titulo-verde"> | ||
<div class="small-11 small-offset-1 columns"><a name="res"></a> | <div class="small-11 small-offset-1 columns"><a name="res"></a> | ||
Line 301: | Line 358: | ||
<div class="small-10 columns small-offset-2"> | <div class="small-10 columns small-offset-2"> | ||
<div class="small-10 small-offset-1 columns"> | <div class="small-10 small-offset-1 columns"> | ||
− | <p class="black"> | + | <p class="black">TEXTO AQUI</p> |
</div> | </div> | ||
</div> | </div> | ||
Line 427: | Line 484: | ||
</div> | </div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | + | --> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 18:40, 25 November 2016
AlgAranha Team USP_UNIFESP-Brazil
DESCRIPTION
EXPERIMENTS
PROOF OF CONCEPT
RESULTS