Difference between revisions of "Team:Arizona State"

 
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{{Arizona State}}
 
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                    <img src="https://static.igem.org/mediawiki/2016/8/87/T--Arizona_State--igemlogotransparent2.png" height=220px><br>
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                    <h1> Ringtones</h1>
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                    <h2>Diverse homoserine lactone systems for cellular communication</h2>
  
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        <center><img src="https://static.igem.org/mediawiki/2016/f/f3/T--Arizona_State--ASUfrontpage1.png"></center>
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        <h1 class = "center"> Our Project </h1>
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        <p class = "big home-text">Quorum sensing (QS) allows bacteria to sense the surrounding
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            cell population density and communicate with their neighbors. They do this by producing
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            N-acyl homoserine lactones (AHLs), which can diffuse back into the bacteria at high concentrations.
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            In the cell they bind to receiver proteins, which then activate gene expression in a coordinated manner. There are many natural QS systems, but only four in popular use by synthetic biologists. Some of these systems use AHLs that can activate multiple receiver systems or "crosstalk" between networks. This can cause problems when using QS in higher-level designs. Our project aims to characterize a variety of AHL networks, which will increase the number of functional QS systems with minimal crosstalk in
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            synthetic biology. </p>
  
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            <h2>The ASU Team</h2>
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                <h2>Check out our Twitter!</h2>
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                    Visit our website >
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          <a href = "/Team:Arizona_State/Description"><div class = "col-sm-4 footer-button design">
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              <p>Description</p>
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              <p>Results</p>
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          <a href = "/Team:Arizona_State/Notebook"><div class = "col-sm-4 footer-button notebook">
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              <p>Notebook</p>
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              <p>Safety</p>
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    <a href="#" class="dropbtn">Our Project</a>
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              <p>Parts and Design</p>
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      <a href="#">Our Project</a>
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              <p>Human Practices</p>
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<h2>Our Project:</h2>
 
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<p> The objective of our project is design and test a variety of quorum sensing networks, on a flexible testing platform. On this platform the QS system is separated into two components designated the “Sender” and the “Receiver”. The HSL synthase is expressed in the Sender cell, while the inducible promoter and regulator are carried by a Receiver cell. When the Sender produces a signal, the HSL, it diffuses across cell membranes and activates the Receiver. In our current system, Receivers will express green fluorescent protein (GFP) in response to induction by Senders from different bacterial species. Currently, our team has built 10 senders and 7 receivers, several of which have been shown to be functional in E. coli. Testing for the remaining systems is still underway, and more receivers are still being cloned. </p>
 
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<p> Our iGEM team is investigating the diverse applications that fit with our quorum sensing project. Some of the sub-projects include: investigating the Aub strain, which originates from unidentified soil bacterium, to decipher its organism of origin; making a “super quorum sensing” E. coli that is engineered to respond to wider variety of HSLs; more comprehensive characterization of HSLs produced by our Senders using mass spec;  and building a genetic-based circuit model using Arduino or Logism. The latter is one of our several outreach projects, designed to increase future participation and interest in the ASU iGEM team. </p>
 
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Latest revision as of 20:34, 28 November 2016


Ringtones

Diverse homoserine lactone systems for cellular communication

Our Project

Quorum sensing (QS) allows bacteria to sense the surrounding cell population density and communicate with their neighbors. They do this by producing N-acyl homoserine lactones (AHLs), which can diffuse back into the bacteria at high concentrations. In the cell they bind to receiver proteins, which then activate gene expression in a coordinated manner. There are many natural QS systems, but only four in popular use by synthetic biologists. Some of these systems use AHLs that can activate multiple receiver systems or "crosstalk" between networks. This can cause problems when using QS in higher-level designs. Our project aims to characterize a variety of AHL networks, which will increase the number of functional QS systems with minimal crosstalk in synthetic biology.


The ASU Team

Check out our Twitter!


Description

Results

Notebook

Safety

Parts and Design

Human Practices

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© 2016 ASU iGEM