Difference between revisions of "Team:Peking/Basic Part"

 
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                    <li class="menu-1"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking" >Home</a></li>
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                    <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievements</a>
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                            <li><a href="https://2016.igem.org/Team:Peking/Demonstrate" >Results</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Basic_Part" >Parts</a></li>
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−
                            <li><a href="https://2016.igem.org/Team:Peking/Collaborations" >Collaborations</a></li>
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        </script>
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                            <li><a href="https://2016.igem.org/Team:Peking/Description" >Overview</a></li>
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        <script>
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                            <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Recovery" >Recovery</a></li>
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−
                            <li><a href="https://2016.igem.org/Team:Peking/Secretion" >Secretion</a></li>
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            }
−
                            <li><a href="https://2016.igem.org/Team:Peking/Proof" >Proof and speculation</a></li>
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                        </ul>
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                    </li>
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        <!-- Navigation -->
−
                    <li class="dropdown menu-4"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Modeling</a>
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        <div id="navigation" class="navbar navbar-fixed-top">
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                            <li><a href="https://2016.igem.org/Team:Peking/Model" >Protein polymerization</a></li>
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                <div class="container no-padding">
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                            <li><a href="https://2016.igem.org/Team:Peking/Software" >Software</a></li>
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                        </ul>
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                    </a>
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                    </li>
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                    <div id="logo" style="max-width:170px"><a class="" href="https://2016.igem.org/Team:Peking"></a></div>
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                    <li class="dropdown menu-5"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Practices</a>
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−
                        <ul class="dropdown-menu">
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                    <div class="nav-collapse collapse">
−
                            <li><a href="https://2016.igem.org/Team:Peking/HP/Gold" >Overview</a></li>
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                        <ul class="nav">
−
                            <li><a href="https://2016.igem.org/Team:Peking/HP/311" >Field research</a></li>
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                            <li class="menu-1"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking" >Home</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/HP/questionnaire" >Questionnaire</a></li>
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                            <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievements</a>
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−
                            <li><a href="https://2016.igem.org/Team:Peking/HP/otherHP" >Other work</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Results" >Results</a></li>
−
                        </ul>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Basic_Part" >Parts</a></li>
−
                    </li>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Collaborations" >Collaborations</a></li>
−
                    <li class="menu-6"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Safety" >Safety</a>
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                                </ul>
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                        <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Lab</a>
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                            <ul class="dropdown-menu">
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                            <li class="dropdown menu-3"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project</a>
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                                <li><a class="" href="https://2016.igem.org/Team:Peking/Team" >Team</a></li>
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−
                                <li><a class="" href="https://2016.igem.org/Team:Peking/Attributions" >Attribution</a></li>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Description" >Overview</a></li>
−
                                <li><a class="" href="https://2016.igem.org/Team:Peking/Notebook" >Notebook</a></li>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li>
−
                            </ul>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li>
−
                        </li>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
−
                        <li class="menu-8"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Interlab" >Interlab</a>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Clearance" >Clearance</a></li>
−
                        </li>
+
                                    <li><a href="https://2016.igem.org/Team:Peking/Secretion" >Secretion</a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/Demonstrate" >Final Performance</a></li>
 +
                                </ul>
 +
                            </li>
 +
                            <li class="dropdown menu-4"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Modeling</a>
 +
                                <ul class="dropdown-menu">
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/Model/GelPoint" > Model of Gel Point </a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/Model/MassDistribution" > Model of Mass Distribution</a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/Software" >Software</a></li>
 +
                                </ul>
 +
                            </li>
 +
                            <li class="dropdown menu-5"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Practices</a>
 +
                                <ul class="dropdown-menu">
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/HP/Gold" >Overview</a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/HP/311" >Field research</a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/HP/questionnaire" >Questionnaire</a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/HP/consulting" >Consulting</a></li>
 +
                                    <li><a href="https://2016.igem.org/Team:Peking/HP/otherHP" >Education&nbsp;&amp;&nbsp;Other</a></li>
 +
                                </ul>
 +
                            </li>
 +
                            <li class="menu-6"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Safety" >Safety</a>
 +
                                <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Lab</a>
 +
                                    <ul class="dropdown-menu">
 +
                                        <li><a class="" href="https://2016.igem.org/Team:Peking/Team" >Team</a></li>
 +
                                        <li><a class="" href="https://2016.igem.org/Team:Peking/Attributions" >Attribution</a></li>
 +
                                        <li><a class="" href="https://2016.igem.org/Team:Peking/Notebook" >Notebook</a></li>
 +
                                    </ul>
 +
                                </li>
 +
                                <li class="menu-8"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Interlab" >Interlab</a>
 +
                                </li>
 +
                                </div>
 +
                </div>
 
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            <h1>Basic Parts<span>.</span></h1>
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                <div class="twelve columns centered text-center">
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            <p class="title1" style="text-align:center"></p>
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                    <h1>Parts</h1>
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        </div>
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                                    <h4><a href="javascript:void(0);" onclick="naver('favor')">Favorite&nbsp;Parts</a></h4>
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                                    <h4><a href="javascript:void(0);" onclick="naver('table')">Part&nbsp;Table</a></h4>
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                            <div class="texttitle">Favorite Parts</div>
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 80% ;" src="https://static.igem.org/mediawiki/2016/6/63/T--Peking--image_partfig1c.png" alt=""/></p>
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                                <figcaption style="text-align:center;">
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                                    Fig. 1. Basic structure of the following parts
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                            <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a></h3>
−
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                                <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/9/94/T--Peking--image_partfig2c.png" alt=""/></p>
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            <ul class="copyright">
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                                <figcaption style="text-align:center;">
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              <!--<li>&copy; 2014 Sparrow</li> -->
+
                                    Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein
−
              <li><a href="2016.igem.org/Team:Peking">Home</a>&nbsp;&nbsp;&nbsp;<a href="mailto:pkuigem2016@126.com">Contact</a></li>
+
                                </figcaption>
−
              <span> &copy;2016 PEKING IGEM. All Rights Reserved.</span>
+
                            </figure>
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              <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li>
+
                            <p class="lead add-bottom" style="color:#5E5656">Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and triple SpyTag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>), our polymer network system is constructed. </p>
−
            </ul>
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                            <br/>
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+
                           
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+
                            <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a></h3>
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/4d/T--Peking--image_partfig3c.png" alt=""/></p>
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  <script>window.jQuery || document.write('<script src="https://2016.igem.org/Template:Peking/Javascript/jquery_1_10_2_min?action=raw&ctype=text/javascript"><\/script>')</script>
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                                <figcaption style="text-align:center;">
−
  <script type="text/javascript" src="https://2016.igem.org/Template:Peking/Javascript/jquery_migrate_1_2_1_min?action=raw&ctype=text/javascript"></script>
+
                                    Fig. 3. 3A: Triple SpyTag mSA: Monomeric streptavidin
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                                </figcaption>
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  <script src="https://2016.igem.org/Template:Peking/Javascript/jquery_flexslider?action=raw&ctype=text/javascript"></script>
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                            </figure>
−
  <script src="https://2016.igem.org/Template:Peking/Javascript/doubleaptogo?action=raw&ctype=text/javascript"></script>
+
                            <p class="lead add-bottom" style="color:#5E5656">Triple SpyCatcher was spaced by 15*(VPGVG). Paired with triple SpyTag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>) and triple SpyTag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>), our polymer network system is constructed.</p>
−
  <script src="https://2016.igem.org/Template:Peking/Javascript/init?action=raw&ctype=text/javascript"></script>  
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<!--quotations from black: start-->
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                            <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a></h3>
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery?action=raw&ctype=text/javascript"></script>
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                            <hr style="border:2px dashed; height:2px" color="#666666">
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<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_sticky?action=raw&ctype=text/javascript"></script>      
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                            <figure>
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_easing_1_3_pack?action=raw&ctype=text/javascript"></script>
+
                                <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/43/T--Peking--image_partfig4c.png" alt=""/></p>
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/bootstrap_min?action=raw&ctype=text/javascript"></script>
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                                <figcaption style="text-align:center;">
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_patallax_1_1_3?action=raw&ctype=text/javascript"></script>
+
                                    Fig. 4. 3B: Triple SpyCatcher
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/appear?action=raw&ctype=text/javascript"></script>
+
                                </figcaption>
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/modernizr?action=raw&ctype=text/javascript"></script>
+
                            </figure>
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_prettyPhoto?action=raw&ctype=text/javascript"></script>
+
                            <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple SpyCatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and triple SpyTag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>), our polymer network system is constructed.</p>
−
<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/isotope?action=raw&ctype=text/javascript"></script>
+
                           
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<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_bxslider_min?action=raw&ctype=text/javascript"></script>
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<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_cycle_all?action=raw&ctype=text/javascript" ></script>
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<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_maximage?action=raw&ctype=text/javascript"></script>
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                            <br/>
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<script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/scripts?action=raw&ctype=text/javascript "></script>
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−
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                            <div class="texttitle">Part Table</div>
−
</body>
+
                           
 +
                            <div class="highlight">
 +
                                </p>
 +
                                <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading…..</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM2016', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
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                                <p>
 +
                            </div>
 +
                           
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                            <a id="collection"></a>
 +
                            <div class="texttitle">Part Collection</div>
 +
                            <p class="lead add-bottom" style="color:#5E5656">Members of Peking iGEM 2016 team  devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.</p>
 +
                            <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM teams.</p>
 +
                           
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                        <!--<li>&copy; 2014 Sparrow</li> -->
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                        <span> &copy;2016 PEKING IGEM. All Rights Reserved.</span>
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Latest revision as of 11:49, 29 November 2016

Parts

Parts

Favorite Parts

Fig. 1. Basic structure of the following parts

BBa_K1989000

Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein

Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (BBa_K1989001) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.


BBa_K1989001

Fig. 3. 3A: Triple SpyTag mSA: Monomeric streptavidin

Triple SpyCatcher was spaced by 15*(VPGVG). Paired with triple SpyTag with SUP (BBa_K1989000) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.

BBa_K1989002

Fig. 4. 3B: Triple SpyCatcher

Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple SpyCatcher (BBa_K1989001) and triple SpyTag with SUP (BBa_K1989000), our polymer network system is constructed.


Part Table

Loading…..

Part Collection

Members of Peking iGEM 2016 team devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.

Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM teams.