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| − | <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievements</a> | + | } |
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| − | <li><a href="https://2016.igem.org/Team:Peking/Basic_Part" >Parts</a></li> | + | |
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| − | <li><a href="https://2016.igem.org/Team:Peking/Model" > Protein polymerization</a></li> | + | <div class="container no-padding"> |
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| − | <li><a href="https://2016.igem.org/Team:Peking/HP/Gold" >Overview</a></li> | + | |
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| − | <li class="menu-6"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Safety" >Safety</a> | + | </ul> |
| − | <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Lab</a> | + | </li> |
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| − | <li><a class="" href="https://2016.igem.org/Team:Peking/Notebook" >Notebook</a></li> | + | <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li> |
| − | </ul>
| + | <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li> |
| − | </li>
| + | <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li> |
| − | <li class="menu-8"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Interlab" >Interlab</a> | + | <li><a href="https://2016.igem.org/Team:Peking/Clearance" >Clearance</a></li> |
| − | </li>
| + | <li><a href="https://2016.igem.org/Team:Peking/Secretion" >Secretion</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/ Demonstrate" >Final Performance</a></li> |
| | + | </ul> |
| | + | </li> |
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| | + | <li><a href="https://2016.igem.org/Team:Peking/Model/MassDistribution" > Model of Mass Distribution</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/Software" >Software</a></li> |
| | + | </ul> |
| | + | </li> |
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| | + | <li><a href="https://2016.igem.org/Team:Peking/HP/311" >Field research</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/HP/questionnaire" >Questionnaire</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/HP/consulting" >Consulting</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/HP/otherHP" > Education & Other</a></li> |
| | + | </ul> |
| | + | </li> |
| | + | <li class="menu-6"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Safety" >Safety</a> |
| | + | <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Lab</a> |
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| | + | <li><a class="" href="https://2016.igem.org/Team:Peking/Team" >Team</a></li> |
| | + | <li><a class="" href="https://2016.igem.org/Team:Peking/Attributions" >Attribution</a></li> |
| | + | <li><a class="" href="https://2016.igem.org/Team:Peking/Notebook" >Notebook</a></li> |
| | + | </ul> |
| | + | </li> |
| | + | <li class="menu-8"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Interlab" >Interlab</a> |
| | + | </li> |
| | + | </div> |
| | + | </div> |
| | </div> | | </div> |
| | </div> | | </div> |
| − | </div>
| + | <!--/Navigation --> |
| − | </div>
| + | |
| − | <!--/Navigation --> | + | |
| | | | |
| − | <!-- Page Title======================================================================== --> | + | |
| − | | + | <!-- Page Title======================================================================== --> |
| − | | + | |
| − | <div class="twelve columns centered text-center"> | + | |
| − | <h1>Basic Parts</h1>
| + | <div class="twelve columns centered text-center"> |
| − | <p class="title1" style="text-align:center"></p> | + | <h1>Parts</h1> |
| − | </div>
| + | <p class="title1" style="text-align:center"></p> |
| − | </div>
| + | </div> |
| − | </div>
| + | </div> |
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| − | | + | <section> |
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| − | <div class="column full_size"> | + | |
| − | <h5>Part Table </h5> | + | |
| − | <div class="highlight"> | + | |
| − | | + | |
| − | | + | |
| − | | + | <div class=" three columns"> |
| − | </p> | + | <div id="page-wrap"> |
| − | <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading…..</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM2016', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script> | + | <div id="sidebar" style="color:#000000"> |
| − | <p> | + | <h4><a href="javascript:void(0);" onclick="naver('favor')">Favorite Parts</a></h4> |
| − | </div> | + | <h4><a href="javascript:void(0);" onclick="naver('table')">Part Table</a></h4> |
| − | </div> | + | <h4><a href="javascript:void(0);" onclick="naver('collection')">Part Collection</a></h4> |
| − | | + | </div> |
| − | </div><!--row end-->
| + | </div> |
| − | </section>
| + | </div> |
| − | </div><!--12 columes end-->
| + | |
| − | </div><!--page-content end--> | + | |
| − | | + | <div class=" nine columns"> |
| − | | + | <a id="favor"></a> |
| − | | + | <div class="texttitle">Favorite Parts</div> |
| − | | + | <br/> |
| − | | + | <figure> |
| − | | + | <p style="text-align:center;"><img style="width: 80% ;" src="https://static.igem.org/mediawiki/2016/6/63/T--Peking--image_partfig1c.png" alt=""/></p> |
| − | | + | <figcaption style="text-align:center;"> |
| − | | + | Fig. 1. Basic structure of the following parts |
| − | <!-- footer============================================================================== --> | + | </figcaption> |
| − | <style> | + | </figure> |
| − | footer .copyright span:before { | + | |
| − | content: "|";
| + | <br/> |
| − | padding-left: 10px;
| + | <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a></h3> |
| − | padding-right: 12px;
| + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| − | color: #b4bbbb;
| + | <figure> |
| − | } | + | <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/9/94/T--Peking--image_partfig2c.png" alt=""/></p> |
| − | footer .copyright span:after { | + | <figcaption style="text-align:center;"> |
| − | content: "|";
| + | Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein |
| − | padding-left: 10px;
| + | </figcaption> |
| − | padding-right: 12px;
| + | </figure> |
| − | color: #b4bbbb;
| + | <p class="lead add-bottom" style="color:#5E5656">Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and triple SpyTag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>), our polymer network system is constructed. </p> |
| − | } | + | <br/> |
| − | </style> | + | |
| − | <footer id="page-footer">
| + | |
| − | | + | |
| − | <div class="twelve columns" > | + | <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a></h3> |
| − | | + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| − | <!--<li>© 2014 Sparrow</li> -->
| + | <figure> |
| − | <li><a href="2016.igem.org/Team:Peking">Home</a> <a href="mailto:pkuigem2016@126.com">Contact</a></li> | + | <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/4d/T--Peking--image_partfig3c.png" alt=""/></p> |
| − | <span> ©2016 PEKING IGEM. All Rights Reserved.</span>
| + | <figcaption style="text-align:center;"> |
| − | <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li> | + | Fig. 3. 3A: Triple SpyTag mSA: Monomeric streptavidin |
| − | </ul>
| + | </figcaption> |
| − | </div>
| + | </figure> |
| − | <div id="go-top" style="display: block;"><a title="Back to Top" href="#">Go To Top</a></div> | + | <p class="lead add-bottom" style="color:#5E5656">Triple SpyCatcher was spaced by 15*(VPGVG). Paired with triple SpyTag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>) and triple SpyTag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>), our polymer network system is constructed.</p> |
| − | </div>
| + | |
| − | </footer> <!-- Footer End-->
| + | |
| − | | + | |
| − | | + | <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a></h3> |
| − | <!-- Java Script======================================================================= --> | + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| − | <script>window.jQuery || document.write('<script src="https://2016.igem.org/Template:Peking/Javascript/jquery_1_10_2_min?action=raw&ctype=text/javascript"><\/script>')</script>
| + | <figure> |
| − | <script type="text/javascript" src="https://2016.igem.org/Template:Peking/Javascript/jquery_migrate_1_2_1_min?action=raw&ctype=text/javascript"></script>
| + | <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/43/T--Peking--image_partfig4c.png" alt=""/></p> |
| − | | + | <figcaption style="text-align:center;"> |
| − | <script src="https://2016.igem.org/Template:Peking/Javascript/jquery_flexslider?action=raw&ctype=text/javascript"></script>
| + | Fig. 4. 3B: Triple SpyCatcher |
| − | <script src="https://2016.igem.org/Template:Peking/Javascript/doubleaptogo?action=raw&ctype=text/javascript"></script>
| + | </figcaption> |
| − | <script src="https://2016.igem.org/Template:Peking/Javascript/init?action=raw&ctype=text/javascript"></script>
| + | </figure> |
| − | | + | <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple SpyCatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and triple SpyTag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>), our polymer network system is constructed.</p> |
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| − | <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/bootstrap_min?action=raw&ctype=text/javascript"></script> | + | <div class="texttitle">Part Table</div> |
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| − | <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_prettyPhoto?action=raw&ctype=text/javascript"></script> | + | <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading…..</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM2016', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script> |
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| − | </body> | + | <a id="collection"></a> |
| | + | <div class="texttitle">Part Collection</div> |
| | + | <p class="lead add-bottom" style="color:#5E5656">Members of Peking iGEM 2016 team devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.</p> |
| | + | <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM teams.</p> |
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