Difference between revisions of "Team:Peking/Basic Part"

 
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                    <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievements</a>
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                            <li><a href="https://2016.igem.org/Team:Peking/Description" >Overview</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Description" >Overview</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Clearance" >Clearance</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Model" >Protein polymerization</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Model/GelPoint" > Model of Gel Point </a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/HP/311" >Field research</a></li>
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                                <li><a class="" href="https://2016.igem.org/Team:Peking/Attributions" >Attribution</a></li>
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                                        <li><a class="" href="https://2016.igem.org/Team:Peking/Notebook" >Notebook</a></li>
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                        <li class="menu-8"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Interlab" >Interlab</a>
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                                <li class="menu-8"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Interlab" >Interlab</a>
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                             <div class="texttitle">Favorite Parts</div>
 
                             <div class="texttitle">Favorite Parts</div>
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                             <br/>
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                             <h3 class="classic-title">Basic structure of the following parts</h3>
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                             <figure>
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                             <hr style="border:2px dashed; height:2px" color="#666666">
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                                <p style="text-align:center;"><img style="width: 80% ;" src="https://static.igem.org/mediawiki/2016/6/63/T--Peking--image_partfig1c.png" alt=""/></p>
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                            <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/c/c5/T—Peking--image_partfig1.png" alt=""/></p>
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                                <figcaption style="text-align:center;">
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                                    Fig. 1. Basic structure of the following parts
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                                </figcaption>
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                            </figure>
 
                              
 
                              
 
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                             <h3 class="classic-title">BBa_K1989000</h3>
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                             <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a></h3>
 
                             <hr style="border:2px dashed; height:2px" color="#666666">
 
                             <hr style="border:2px dashed; height:2px" color="#666666">
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                          <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/e/e9/T--Peking--image_partfig2.png" alt=""/></p>
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/9/94/T--Peking--image_partfig2c.png" alt=""/></p>
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                             <p class="lead add-bottom" style="color:#5E5656">Super uranyl-binding protein (SUP) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the uranyl-binding capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with mSA (BBa_K1989002) our hydrogel system is constructed. </p>
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                                <figcaption style="text-align:center;">
 +
                                    Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein
 +
                                </figcaption>
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                            </figure>
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                             <p class="lead add-bottom" style="color:#5E5656">Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and triple SpyTag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>), our polymer network system is constructed. </p>
 
                             <br/>
 
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                             <h3 class="classic-title">BBa_K1989001</h3>
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                             <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a></h3>
 
                             <hr style="border:2px dashed; height:2px" color="#666666">
 
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                             <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/c/c5/T--Peking--image_partfig3.png" alt=""/></p>
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                             <figure>
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                             <p class="lead add-bottom" style="color:#5E5656">Triple spycatcher was spaced by 15*(VPGVG). Paired with triple spytag (BBa_K1989000) and tripled spytag with mSA (BBa_K1989002) our hydrogel system is constructed.</p>
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                                <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/4d/T--Peking--image_partfig3c.png" alt=""/></p>
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                             <h3 class="classic-title">BBa_K1989002</h3>
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                                <figcaption style="text-align:center;">
 +
                                    Fig. 3. 3A: Triple SpyTag mSA: Monomeric streptavidin
 +
                                </figcaption>
 +
                            </figure>
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                             <p class="lead add-bottom" style="color:#5E5656">Triple SpyCatcher was spaced by 15*(VPGVG). Paired with triple SpyTag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>) and triple SpyTag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>), our polymer network system is constructed.</p>
 +
                           
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                             <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a></h3>
 
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                          <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/d/d9/T--Peking--image_partfig4.png " alt=""/></p>
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                            <figure>
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                             <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the recyclable capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with SUP (BBa_K1989000) our hydrogel system is constructed.</p>
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                                <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/43/T--Peking--image_partfig4c.png" alt=""/></p>
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                                <figcaption style="text-align:center;">
 +
                                    Fig. 4. 3B: Triple SpyCatcher
 +
                                </figcaption>
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                            </figure>
 +
                             <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple SpyCatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and triple SpyTag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>), our polymer network system is constructed.</p>
 
                              
 
                              
 
                              
 
                              
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                            <p class="lead add-bottom" style="color:#5E5656">2016 Peking iGEM team members devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.</p>
 
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                            <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM team.</p>
 
 
                              
 
                              
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                            <div class="texttitle">Part Collection</div>
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                            <p class="lead add-bottom" style="color:#5E5656">Members of Peking iGEM 2016 team  devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.</p>
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                            <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM teams.</p>
 
                              
 
                              
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Latest revision as of 11:49, 29 November 2016

Parts

Parts

Favorite Parts

Fig. 1. Basic structure of the following parts

BBa_K1989000

Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein

Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (BBa_K1989001) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.


BBa_K1989001

Fig. 3. 3A: Triple SpyTag mSA: Monomeric streptavidin

Triple SpyCatcher was spaced by 15*(VPGVG). Paired with triple SpyTag with SUP (BBa_K1989000) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.

BBa_K1989002

Fig. 4. 3B: Triple SpyCatcher

Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple SpyCatcher (BBa_K1989001) and triple SpyTag with SUP (BBa_K1989000), our polymer network system is constructed.


Part Table

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Part Collection

Members of Peking iGEM 2016 team devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.

Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM teams.