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| <p> | | <p> |
− | <strong>LI Cheng</strong> mainly focused on the overall operating of the team, meanwhile he took a part in measurement of protein function. He was also involved in team interaction and academic communication.<br/> | + | <strong>LI Cheng</strong> mainly focused on the overall operating of the team, meanwhile he took part in the evaluation of protein function. He was also involved in team interaction and academic communication.<br/> |
− | <strong>YANG Xiaoyu</strong> was responsible for protein purification and some evaluation experiments. <br/> | + | <strong>YANG Xiaoyu</strong> was responsible for the plasmid construction at early stage and then mainly focused on the protein purification. He also participated in secretion effect examination. <br/> |
− | | + | |
| <strong>BAI Ke</strong> was in charge of all experiments related to secretion, including plasmid construction, gene expression and secretory protein examination. He is also one of the speakers who give presentation.<br/> | | <strong>BAI Ke</strong> was in charge of all experiments related to secretion, including plasmid construction, gene expression and secretory protein examination. He is also one of the speakers who give presentation.<br/> |
| <strong>DONG Yiming</strong> was responsible for building secretion module, parts and the interlab.<br/> | | <strong>DONG Yiming</strong> was responsible for building secretion module, parts and the interlab.<br/> |
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| <strong>YANG Changru</strong> was mainly responsible for the test experiment (crosslinking) of protein and modeling, as well as designing some plasmids. He also did some quantitative experiments of uranyl absorption and secretion with the cooperation of teammates.<br/> | | <strong>YANG Changru</strong> was mainly responsible for the test experiment (crosslinking) of protein and modeling, as well as designing some plasmids. He also did some quantitative experiments of uranyl absorption and secretion with the cooperation of teammates.<br/> |
| <strong>ZHAO Shijun</strong> devoted himself to molecular cloning and part construction.<br/> | | <strong>ZHAO Shijun</strong> devoted himself to molecular cloning and part construction.<br/> |
− | <strong>LV Nayun</strong> has taken part in the protein extraction and the analysis of the questionnaire results.<br/> | + | <strong>LYV Nayun</strong> took part in the protein purification and the analysis of the questionnaire results.<br/> |
| <strong>WANG Dingyu</strong> was responsible for protein purification and some art design.<br/> | | <strong>WANG Dingyu</strong> was responsible for protein purification and some art design.<br/> |
| <strong>LI Jiamian</strong> was the art designer and photographer of our team and he participated in wiki building and PowerPoint design.<br/> | | <strong>LI Jiamian</strong> was the art designer and photographer of our team and he participated in wiki building and PowerPoint design.<br/> |
| <strong>WEI Jingyi</strong> was responsible for plasmid construction for Secretion Part.<br/> | | <strong>WEI Jingyi</strong> was responsible for plasmid construction for Secretion Part.<br/> |
| <strong>WANG Yuqing</strong> was the lab manager and in charge of wiki building, she also participated in human practices, art design and part of the experimental work.<br/> | | <strong>WANG Yuqing</strong> was the lab manager and in charge of wiki building, she also participated in human practices, art design and part of the experimental work.<br/> |
− | <strong>TENG Huaiyuan</strong> was in charge of modeling and software design. I studied Flory J.Paul's theory in polymer chemistry and furthermore established the model of molecular weight distribution in our case.<br/> | + | <strong>TENG Huaiyuan</strong> was in charge of modeling and software design. He studied Flory J.Paul's theory in polymer chemistry and furthermore established the model of molecular weight distribution in our case.<br/> |
− | <strong>NING Xin</strong> was mainly responsible for the molecular cloning of the secretion system ,while participating in the measurement of proteins's functions as well.<br/> | + | <strong>NING Xin</strong> was mainly responsible for the molecular cloning of the secretion system, while participating in the measurement of proteins's functions as well.<br/> |
| <strong>CHANG Xuyao</strong> was in charge of plasmid construction of the Secretion Part. | | <strong>CHANG Xuyao</strong> was in charge of plasmid construction of the Secretion Part. |
| </p> | | </p> |
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| <a id="acknowledgement"></a> | | <a id="acknowledgement"></a> |
| <h3 class="classic-title";"><span>Acknowledgement</span></h3> | | <h3 class="classic-title";"><span>Acknowledgement</span></h3> |
− | <p>We'd like to thank all those who have helped us over the summer. Without whose sincere support, the Uranium Reaper would have not been achieved. | + | <p>We'd like to thank all those who have helped us over the summer. Without their sincere support, the Uranium Reaper would have not been achieved. |
| </p> | | </p> |
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| <br> | | <br> |
| <h4>Materials Support</h4> | | <h4>Materials Support</h4> |
− | <p><strong>Dr. ZHANG Wenbing</strong> provided us with the plasmid of 3B(Tri-SpyCatcher within 15X linker).<br> | + | <p><strong>Dr. ZHANG Wenbing</strong> provided us with the plasmid of 3B (Tri-SpyCatcher flanked by 15X linker).<br> |
− | <strong> Prof. ZHOU Lu</strong> provided us with Uranyl-binding Protein (SUP).<br> | + | <strong> Prof. ZHOU Lu</strong> provided us with Super Uranyl-binding Protein (SUP).<br> |
| <strong>Prof. LIU Chunli</strong> allowed us to use his lab to do experiments on uranyl ion. The uranyl nitrate solution and Arsenazo III was provided by Prof. LIU.<br> | | <strong>Prof. LIU Chunli</strong> allowed us to use his lab to do experiments on uranyl ion. The uranyl nitrate solution and Arsenazo III was provided by Prof. LIU.<br> |
| <strong>Prof. LOU Chunbo</strong> provided us with <i>B.subtilis</i>, gene sequence of mSA, and gene sequences of heavy metal ions binding proteins (lead-binding protein, mercury-binding protein and cadmium-binding protein).<br> | | <strong>Prof. LOU Chunbo</strong> provided us with <i>B.subtilis</i>, gene sequence of mSA, and gene sequences of heavy metal ions binding proteins (lead-binding protein, mercury-binding protein and cadmium-binding protein).<br> |
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| <strong>Prof. CHANG Zengyi</strong> and <strong>Dr. WANG Qingsong</strong> provided us with the scanner and helped with the setup and scan of gels.<br> | | <strong>Prof. CHANG Zengyi</strong> and <strong>Dr. WANG Qingsong</strong> provided us with the scanner and helped with the setup and scan of gels.<br> |
| <strong>Prof. LIU Chunli</strong> provided us with Geiger counter to test the radiation level in our lab.<br> | | <strong>Prof. LIU Chunli</strong> provided us with Geiger counter to test the radiation level in our lab.<br> |
− | <strong>Core Facilities</strong>at School of Life Sciences, Peking University, assisted us with ITC.<br> | + | <strong>Core Facilities</strong> at School of Life Sciences, Peking University, assisted us with ITC.<br> |
| <strong>The Testing center of College of Environmental Sciences and Engineering, PKU</strong> provided the Aurora M90 mass spectrograph. | | <strong>The Testing center of College of Environmental Sciences and Engineering, PKU</strong> provided the Aurora M90 mass spectrograph. |
| </p> | | </p> |
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| <strong>Prof. ZHANG Wenbing, Prof. LOU Chunbo</strong> and <strong>Dr. YU Daqi</strong> kindly helped our team with modeling of the project.<br> | | <strong>Prof. ZHANG Wenbing, Prof. LOU Chunbo</strong> and <strong>Dr. YU Daqi</strong> kindly helped our team with modeling of the project.<br> |
| <strong>Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian</strong> and <strong>ZHANG Yihao</strong> coached us for the presentation.<br> | | <strong>Prof. OUYANG Qi, Prof. LOU Chunbo, Dr. ZHANG Haoqian</strong> and <strong>ZHANG Yihao</strong> coached us for the presentation.<br> |
− | <strong>LI Ying</strong> and <strong>ZHANG Xueping</strong> provided us with kind suggestions of our presentation.<br> | + | <strong>LI Ying</strong> and <strong>ZHANG Xueping</strong> provided us with kind suggestions for our presentation.<br> |
− | <strong>Prof. LAI Luhua, Prof. TANG Chao, Prof. CHEN Xing, Prof. CHEN Peng</strong> and <strong>Prof. LI Chenjian</strong> participated in our presentation rehearsal and gave suggestions for revisions.<br> | + | <strong>Prof. LI Chenjian, Prof. TANG Chao, Prof. CHEN Xing, Prof. CHEN Peng</strong> and <strong>Prof. LAI Luhua</strong> participated in our presentation rehearsal and gave suggestions for revisions.<br> |
| <strong><a href="http://yiwen.ba">Yiwen Expert Text Solutions (yiwen.ba)</a></strong> provided expert text correction service for the content of our wiki.<br> | | <strong><a href="http://yiwen.ba">Yiwen Expert Text Solutions (yiwen.ba)</a></strong> provided expert text correction service for the content of our wiki.<br> |
| <strong>NWPU</strong> offered us helpful suggestions of PowerPoint. | | <strong>NWPU</strong> offered us helpful suggestions of PowerPoint. |