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| </figure> | | </figure> |
| <figure> | | <figure> |
− | <p style="text-align:center;"><img style="width:52%;" src="https://static.igem.org/mediawiki/2016/1/19/Fig2%E6%94%B9.png" alt=""/></p> | + | <p style="text-align:center;"><img style="width:52%;" src="https://static.igem.org/mediawiki/2016/8/8d/T--Peking--images_FP_fig2zhuzhuangtu.png" alt=""/></p> |
| <figcaption style="text-align:left;"> | | <figcaption style="text-align:left;"> |
| Fig. 2. The remaining proportion of 10μM uranyl in TBS buffer, fresh and seawater after treatment with our polymer network. ****p < 0.0001. n=3. Error bars indicate standard deviations. | | Fig. 2. The remaining proportion of 10μM uranyl in TBS buffer, fresh and seawater after treatment with our polymer network. ****p < 0.0001. n=3. Error bars indicate standard deviations. |
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| <a id="Conclusion"></a> | | <a id="Conclusion"></a> |
| <div class="texttitle">Discussion</div> | | <div class="texttitle">Discussion</div> |
− | <p>In this integrated experiment, the Uranium Reaper showed an adsorption rate of 60%, which corresponds well to the product of the uranyl adsorption rate and polymer network recovery rate (90% × 70% = 63%). This result has reached the expectations but is still far removed from the efficiencies that would be needed for an industrial process. Consequently, both the uranyl adsorption and polymer network recovery rate have to be increased to improve the overall efficiency of the Uranium Reaper, which, of course, requires further research and development.</p> | + | <p class="lead add-bottom" style="color:#5E5656">In this integrated experiment, the Uranium Reaper showed an adsorption rate of 60%, which corresponds well to the product of the uranyl adsorption rate and polymer network recovery rate (90% × 70% = 63%). This result has reached the expectations but is still far removed from the efficiencies that would be needed for an industrial process. Consequently, both the uranyl adsorption and polymer network recovery rate have to be increased to improve the overall efficiency of the Uranium Reaper, which, of course, requires further research and development.</p> |
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| <a id="App"></a> | | <a id="App"></a> |
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| <figure> | | <figure> |
− | <p style="text-align:center;"><img style="width:82% ;" src="https://static.igem.org/mediawiki/2016/c/c8/T--Peking--images_FP_fig3.png" alt=""/></p> | + | <p style="text-align:center;"><img style="width:80% ;" src="https://static.igem.org/mediawiki/2016/c/c8/T--Peking--images_FP_fig3.png" alt=""/></p> |
| <figcaption style="text-align:left;"> | | <figcaption style="text-align:left;"> |
| Fig. 3. The adsorption of Cd (A) and Pb (B) by modified functional polymer network based on the Uranium Reaper platform. | | Fig. 3. The adsorption of Cd (A) and Pb (B) by modified functional polymer network based on the Uranium Reaper platform. |
| </figcaption> | | </figcaption> |
| </figure> | | </figure> |
− | <p class="lead add-bottom" style="color:#5E5656">We simulated Cd and Pb pollution (10μM) in TBS buffer. Using the same strategy which we have used for uranyl removal, with the 3A-SUP monomer replaced by 3A-CBP or 3A-LBP, about 85% of the Cd 55% of the Pb could be adsorbed, respectively. The results demonstrated that the strategy has great potential for broader metal recovery applications. It is thus necessary to optimize the polymer network under various conditions.</p> | + | <p class="lead add-bottom" style="color:#5E5656">We simulated Cd and Pb pollution (10μM) in TBS buffer. Using the same strategy which we have used for uranyl removal, with the 3A-SUP monomer replaced by 3A-CBP or 3A-LBP, about 84.5% of the Cd 53.9% of the Pb could be adsorbed, respectively. The results demonstrated that the strategy has great potential for broader metal recovery applications. It is thus necessary to optimize the polymer network under various conditions.</p> |
| <h3 class="classic-title" id="Visual">Visualization</h3> | | <h3 class="classic-title" id="Visual">Visualization</h3> |
| <p class="lead add-bottom" style="color:#5E5656">We fused mRFP to the Triple SpyTag to make impart color to our polymer network (Fig. 4.).</p> | | <p class="lead add-bottom" style="color:#5E5656">We fused mRFP to the Triple SpyTag to make impart color to our polymer network (Fig. 4.).</p> |
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| </figcaption> | | </figcaption> |
| </figure> | | </figure> |
− | <p class="lead add-bottom" style="color:#5E5656">When the SUP module in the Triple SpyTag-SUP monomer was changed to mRFP, a red-colored gel was formed (Fig 2. left), and by crosslinking proteins at higher concentrations, we could get a relatively stable gel structure. This attribute of the polymer network promises a literally colorful future in artistic creation, with potential applications in 3D printing.</p> | + | <p class="lead add-bottom" style="color:#5E5656">When the SUP module in the Triple SpyTag-SUP monomer was changed to mRFP, a red-colored gel was formed (Fig 4. left), and by crosslinking proteins at higher concentrations, we could get a relatively stable gel structure. This attribute of the polymer network promises a literally colorful future in artistic creation, with potential applications in 3D printing.</p> |
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