Difference between revisions of "Team:USP UNIFESP-Brazil"

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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<img style="max-height: 120px;" src="https://static.igem.org/mediawiki/2016/thumb/f/f6/T--USP_UNIFESP-Brazil--LOGO.png/370px-T--USP_UNIFESP-Brazil--LOGO.png" />
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<p>AlgAranha Team USP_UNIFESP-Brazil</p>
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<p style="text-align: right; padding-right: 10.5%;"><a href="https://2016.igem.org/">iGEM 2016</a></p>
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<li><a href="https://2016.igem.org/wiki/index.php?title=Team:USP_UNIFESP-Brazil" >Home</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Team">Team</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Project" >Project</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Parts">Parts</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Interlab">Interlab</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Human_Practices">Human Practices</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Awards">Awards</a></li>
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<li><a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Attributions">Attributions</a></li>
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<h5> Team Description </h5>
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<p> We are a multidisciplinary team from São Paulo, Brasil, with students of architecture, biology, biomedical sciences, social sciences and more,from the universities USP, UNESP and UNIFESP. The team originated from the synthetic biology club (<a href =https://s3.synbiobrasil.org/>SynBio Brasil </a>), which is a independent club that works promoting synbio and open science awereness and education. Since 2013, different members of the club have organized themselves to take part in iGEM competition .</p>
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<p>This year, our project is based on the heterologous expression of spider silk protein in <i> Chlamydomonas reinhardtii </i>. The project started when we looked at the problem of growing antibiotic resistance and started to think in ways to tackle it. We specially focused on injury related infections, for example in the case of burn victms. We devised the creation of an antibiotic patch, combining the physical properties of spider silk with antibiotic enzymes (enzybiotics). We intend to express both the spider silk and chimeric enzybiotic proteins with spider silk motifs in <i> Chlamydomonas </i> and polymerize then together to form the product of interest. We hope to acomplish, besides the final goal of patch development, improvement of  <i> Chlamydomonas </i> as a synbio chassis and analysis of its capability of producing enzybiotics and monomers of spider silk. Moreover, the team is envolved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public.      </p>  
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<h2> AlgAranha </h2>
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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Unfortunately, we were not able to achieve the end goal of silk production in <i> Chlamydomonas reinhardtii</i>, but we managed to do some nice things (and get a SILVER MEDAL!)
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Here you can find stuff about:
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</p>
  
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<h5> Editing your wiki </h5>
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Our <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Hardware">DIY Centrifuge</a>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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Our efforts to assemble the spider silk genes at the USER multimerization part of our <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Notebook/">lab notebook</a>
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Transforming <i>Chlamydomonas reinhardtii</i> to produce heterologous proteins, with <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Proof">results</a> and <a href="https://2016.igem.org/Team:USP_UNIFESP-Brazil/Notebook">protocols</a>
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<h5>Tips</h5>
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If you're interested in contacting us, don't hesitate! Whether it's answering questions, just chatting (about SynBio or not, your pick!), making plans for the future or inviting us to go grab a coffee, we would be happy to reply. You can do so at our <a href ="https://www.facebook.com/iGEMUSPUNIFESP2016/">Facebook page</a>or by emailing us at <u><b>igemsp2016@gmail.com</u></b>. Checkout our <a href ="https://www.youtube.com/watch?v=i5yGrCJ7awo">campaign video</a>!
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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We are a multidisciplinary team from São Paulo, Brazil, with students from different majors, as architecture, biology, biomedical sciences, social sciences, and also from the universities USP, UNESP and UNIFESP. The team was originated from the synthetic biology club <a href ="https://s3.synbiobrasil.org/"> (SynBio Brasil), </a> which is an independent group that works promoting synbio and open science awareness and education. Since 2012, different club members have organized themselves to take part in iGEM competition.
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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This year, our project was based on the heterologous expression of spider silk protein in the microalgae <i> Chlamydomonas reinhardtii</i>. We named it AlgAranha, a combination of the portuguese words for algae and spider. Besides the goal of producing enzybiotics and monomers of spider silk, we aim to achieve an improvement of  <i> Chlamydomonas </i> as a synbio chassis.<!--The project started when we looked at the problem of growing antibiotic resistance and started to think in ways to tackle it. We specially focused on injury related infections, for example in the case of burn victms. We devised the creation of an antibiotic patch, combining the spider silk physical properties with antibiotic enzymes (enzybiotics). We intend to express both the spider silk and chimeric enzybiotic proteins with spider silk motifs in <i> Chlamydomonas </i> and polymerize them together to form the product of interest. We hope to accomplish, besides the final goal of patch development, improvement of  <i> Chlamydomonas </i> as a synbio chassis and analysis of its capability of producing enzybiotics and monomers of spider silk.--> Moreover, the team is involved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public.      </p>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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<img src="https://static.igem.org/mediawiki/2016/6/6d/T--USP_UNIFESP-Brazil--chlamydomonas2-1.jpg" style="margin-bottom:20px;margin-top:0px;margin-left:180px"/>
  
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Scanning electron microscope image, showing <i>Chlamydomonas reinhardtii</i>, a unicellular flagellate used as a model system in molecular genetics work and flagellar motility studies. Author: Dartmouth Electron Microscope Facility, Dartmouth College
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<h4> Help the team! </h4>
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<p><a href="http://www.vakinha.com.br/vaquinha/brasileiros-pesquisam-supercurativo-para-vitimas-de-queimaduras">Brazilian campaign</a></p>
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<p><a href=" http://go.dodofunding.com/campaigns/algaranha/">International campaign</a></p>
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Latest revision as of 23:38, 2 December 2016

AlgAranha Team USP_UNIFESP-Brazil

iGEM 2016



Unfortunately, we were not able to achieve the end goal of silk production in Chlamydomonas reinhardtii, but we managed to do some nice things (and get a SILVER MEDAL!) Here you can find stuff about:

Our DIY Centrifuge

Our efforts to assemble the spider silk genes at the USER multimerization part of our lab notebook

Transforming Chlamydomonas reinhardtii to produce heterologous proteins, with results and protocols

If you're interested in contacting us, don't hesitate! Whether it's answering questions, just chatting (about SynBio or not, your pick!), making plans for the future or inviting us to go grab a coffee, we would be happy to reply. You can do so at our Facebook pageor by emailing us at igemsp2016@gmail.com. Checkout our campaign video!

We are a multidisciplinary team from São Paulo, Brazil, with students from different majors, as architecture, biology, biomedical sciences, social sciences, and also from the universities USP, UNESP and UNIFESP. The team was originated from the synthetic biology club (SynBio Brasil), which is an independent group that works promoting synbio and open science awareness and education. Since 2012, different club members have organized themselves to take part in iGEM competition.

This year, our project was based on the heterologous expression of spider silk protein in the microalgae Chlamydomonas reinhardtii. We named it AlgAranha, a combination of the portuguese words for algae and spider. Besides the goal of producing enzybiotics and monomers of spider silk, we aim to achieve an improvement of Chlamydomonas as a synbio chassis. Moreover, the team is involved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public.