Wednesday 20^th July

Lab work

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Biobrick characterization

pclTAA, pclTAG and pclTq transformations

By Laetitia and Caroline

In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pclTAA, pclTAG and pclTq. A control was made with cells without plasmids. The transformed bacteria resulting will be put on glycerol. Cells were plated on LB + Streptomycin (50µg/ml) and incubated overnight at 37°C.

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Visualization

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Get DNA Closer

Electrophoresis of PCR products DS_SPcasN, DS_TDcasN and pZA21

By Caroline

PCR products obtained the 19/07/2016 were put to migrate for 30min in a 0,8% agarose gel.

pZA21 transformation

By Laetitia and Caroline

In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pZA21. A control was made with cells without plasmids. The transformed bacteria resulting will be put on glycerol. Cells were plated on LB + Kanamycin (50µg/ml) and incubated overnight at 37°C.