Difference between revisions of "Team:Paris Saclay/Notebook/June/28"
(→pUC19-gBlocks ligation (cf. protocol)) |
(→Interlab study) |
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<!---picture---> | <!---picture---> | ||
| − | |||
===Visualization=== | ===Visualization=== | ||
====pUC19 digestion==== | ====pUC19 digestion==== | ||
| − | pUC19 was digested | + | pUC19 was digested by HincII. A fragment of 2.7kb (linearized plasmid) is expected. |
| − | [[File:T-- | + | |
| + | [[File:T--Paris_Saclay--160628_visualization_pUC19digestion_echelle.jpg|400px|thumb|right|Migration of pUC19 (digested)]] | ||
====pUC19-gBlocks ligation (cf. [[Team:Paris_Saclay/Experiments#Ligation|protocol]])==== | ====pUC19-gBlocks ligation (cf. [[Team:Paris_Saclay/Experiments#Ligation|protocol]])==== | ||
| Line 86: | Line 86: | ||
!'''Plasmid name''' | !'''Plasmid name''' | ||
|pPS16_001<div id="pPS16_001"></div> | |pPS16_001<div id="pPS16_001"></div> | ||
| − | |pPS16_002 | + | |pPS16_002<div id="pPS16_002"></div> |
| − | |pPS16_003 | + | |pPS16_003<div id="pPS16_003"></div> |
| − | |pPS16_005 | + | |pPS16_005<div id="pPS16_005"></div> |
| − | |pPS16_006 | + | |pPS16_006<div id="pPS16_006"></div> |
| − | |pPS16_007 | + | |pPS16_007<div id="pPS16_007"></div> |
| − | |pPS16_008 | + | |pPS16_008<div id="pPS16_008"></div> |
| − | |pPS16_009 | + | |pPS16_009<div id="pPS16_009"></div> |
|} | |} | ||
| + | |||
| + | The gBlock 2.2 was not avaiblable at the time, that is why it was not transformed with the orthers. But when it will be received it will inserted in puc19 and name after pPS16_004 <div id="pPS16_004"></div>. | ||
====Transformation with ligation products==== | ====Transformation with ligation products==== | ||
| Line 122: | Line 124: | ||
''By Caroline'' | ''By Caroline'' | ||
| − | A colony of BL21 cells was put into 4mL of LB medium and incubated overnight at 37°C, 180 rpm. | + | A colony of BL21 cells was put into 4mL of LB medium and incubated overnight at 37°C, 180 rpm. For this part of the project, we will used BL21 e. coli strain because K1372001 contained a tRNAsup for UAG which DH5alp has naturally. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 14:17, 25 July 2016
