Difference between revisions of "Team:Paris Saclay/Notebook/July/27"

(Created page with "{{Team:Paris_Saclay/notebook_header}} =Wednesday 27<sup>th</sup> July= ==Lab work== ===Visualization=== ======== ''By '' ===Biobrick Characterization=== ====BL21 electrocomp...")
 
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''By Charlène''
 
''By Charlène''
  
We obtained transformed bacteria for every conditions made yesterday (except for controls).  
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We obtained transformed bacteria for every conditions made yesterday (except for controls).
 +
File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg
 +
 
We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.  
 
We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.  
  

Revision as of 12:33, 27 July 2016

Wednesday 27th July

Lab work

Visualization

==

By


Biobrick Characterization

BL21 electrocompetent cells preparation for glycerol preparation

By Charlène

We obtained transformed bacteria for every conditions made yesterday (except for controls). File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg

We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.