Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
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[[File:T--Paris_Saclay--160711_characterization_K13-TAG.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_TAG and K1372001]] | [[File:T--Paris_Saclay--160711_characterization_K13-TAG.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_TAG and K1372001]] | ||
[[File:T--Paris_Saclay--160711_characterization_K13-Tq.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_Tq and K1372001]] | [[File:T--Paris_Saclay--160711_characterization_K13-Tq.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_Tq and K1372001]] | ||
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We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm. | We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm. | ||
Revision as of 12:45, 27 July 2016
