Wednesday 27^th July

Lab work

Visualization

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Biobrick Characterization

BL21 electrocompetent cells preparation for glycerol preparation

By Charlène

We obtained transformed bacteria for every conditions made yesterday (except for controls).

Colonies of BL21 transformed with pcl_TAA and K1372001

Colonies of BL21 transformed with pcl_TAG and K1372001

Colonies of BL21 transformed with pcl_Tq and K1372001

We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.