Difference between revisions of "Team:Paris Saclay/Notebook/July/28"
(→Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009) |
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''By Laetitia '' | ''By Laetitia '' | ||
| − | PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. | + | PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. |
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Thus, the PCR mix was done for 12 tubes following the usual protocol. | Thus, the PCR mix was done for 12 tubes following the usual protocol. | ||
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Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. | Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. | ||
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PCR was done with a Tm at 57°C. | PCR was done with a Tm at 57°C. | ||
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Each PCR Product was put to migrate on an agarose gel. | Each PCR Product was put to migrate on an agarose gel. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 10:00, 28 July 2016
