Wednesday 27^th July

Lab work

Visualization

Transformation of DH5a cells

By Léa

DH5a were transformed with pPS16_001, pPS16_002, pPS16_005, or pPS16_009 using the usual protocol. pPS16_002 ligation products of the 19th of July and the 27th of July were used. pPS16_001, pPS16_005 and pPS16_009 ligation products of the 26th of July were used. The Heat shock were performed at 42°C during 60 seconds.

After a one hour incubation, transformation products were streaked on Petri dishes containing Ampicilin, X-Gal and IPTG, and incubated at 37°C overnight.

gBlock 1.2 insertion in puc19

By Caroline

The insertion was carried out following the usual protocol.

Biobrick Characterization

BL21 electrocompetent cells preparation for glycerol preparation

By Charlène

We obtained transformed bacteria for every conditions made yesterday (except for controls).

Colonies of BL21 transformed with pcl_TAA and K1372001

Colonies of BL21 transformed with pcl_TAG and K1372001

Colonies of BL21 transformed with pcl_Tq and K1372001

We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. 3 clones of each condition were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm.