Monday 27^th June

Lab Meeting

Members present:

• Instructors and advisors: Claire, Fabio, Marie, Olivier, Sylvie, Philippe.
• Students: Carla, Caroline, Charlène, Claire, Coline, Léa, Mahnaz, Marion, Martin, Maxence, Naiane, Yacine

Labwork

Interlab study

Cell culture

By Lea

Transformed bacteria frozen on 24/06/2016 were put into 3mL of LB medium containing 30µg/mL chloramphenicol and incubated overnight at 37°C, 180rpm.

Visualization

BioBrick construction

By Caroline, Charlène and Naïane

GBlocks from IDT were received on the 24/06/2016.

pUC19 plasmid was digested using HincII restriction enzyme.

The mix was incubated for 1 hour at 37°C. Then, 1µL of HincII was added and the mix was incubated for 1 hour at 37°C. An electrophoresis was done (0.8% of agarose).

A DNA-ladder was used to visualize the DNA fragments size.

Bringing DNA closer

Cell culture

By Alice and Lea

4 plasmids containing NM Cas9 (DS-NMcas), SP Cas9 (DS-SPcasN-), ST1 Cas9 (DS-ST1casN-) and Td Cas9 (DS-TDcasN-) were ordered to Addgene and received on 24/06/2016. Plasmids were delivered into tubes containing LB agar medium and bacteria colony containing plasmids. Bacteria were put into 15mL of LB medium containing 50µg/mL of spectinomycin and incubated overnight at 37°C, 180 rpm.

BioBrick characterization

By Alice and Lea

BioBrick [http://parts.igem.org/Part:BBa_K1372001 K1372001] from iGEM team Paris Saclay 2014 was chosen to be characterized. 10µL of DH5α|K1372001 cells were put into 3mL of LB medium containing 30µg/mL of chloramphenicol and incubated overnight at 37°C, 180 rpm.