Difference between revisions of "Team:Paris Saclay/Notebook/August/1"
(→DH5alp|pPS16_003, DH5alp|pPS16_003, DH5alp|pPS16_003 and DH5alp|pPS16_003 streak to recover colonies) |
(→Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19) |
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| Line 12: | Line 12: | ||
gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | ||
| + | For each plasmid we streaked 50µL and 150µL of bacteria on LB + X-Gal + IPTG Petri dishes. | ||
====gBlock 3.1 insertion in puc19==== | ====gBlock 3.1 insertion in puc19==== | ||
Revision as of 14:00, 1 August 2016
