(→DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015) |
(→DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015) |
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Line 9: | Line 9: | ||
Each clone was re-plated and put at 37°C. | Each clone was re-plated and put at 37°C. | ||
− | + | We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm). | |
− | + | ||
==== Sequencing of the gBlock 4.2 ==== | ==== Sequencing of the gBlock 4.2 ==== |
Revision as of 11:37, 2 August 2016