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''By Caroline''
''By Caroline''
− The device 1 was transformed in DH5alp following the usual protocol
+ The device 1 was transformed in DH5alp following the usual Heat-shock [[Team:Paris_Saclay/Experiments#heat-shocktransformation| protocol]]. The transformations were streaked on Petri dishes LB+Amp(50µg/mL)+IPTG(0.1µL/mL)+XGal(0.25µL/mL) and incubated overnight at 37°C.
{{Team:Paris_Saclay/notebook_footer}}
{{Team:Paris_Saclay/notebook_footer}}
Revision as of 14:26, 2 August 2016
Contents
1 Tuesday 2st August
1.1 Lab work
1.1.1 Visualization
1.1.1.1 DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015
1.1.1.2 Sequencing of the gBlock 4.2
1.1.1.3 Culture of DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006, DH5alp|pPS16_007 and DH5alp|pPS16_008
1.1.2 Interlab Study
Tuesday 2st August
Lab work
Visualization
DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015
By Charlène, Mathilde, Laetitia, Caroline and Léa
A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers . 6 clones for each transformations were tested.
Each clone was re-plated and put at 37°C.
We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm).
Sequencing of the gBlock 4.2
By Caroline
The PCR products obtain with Q5 on pPS16_008 were send to sequencing.
Culture of DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006, DH5alp|pPS16_007 and DH5alp|pPS16_008
Bu Caroline
One colonie from each transformation was put into LB + Amp (50µL/mL) and incubated overnight at 37°C.
Interlab Study
Device 1 transformation
By Caroline
The device 1 was transformed in DH5alp following the usual Heat-shock protocol . The transformations were streaked on Petri dishes LB+Amp(50µg/mL)+IPTG(0.1µL/mL)+XGal(0.25µL/mL) and incubated overnight at 37°C.