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| − | <h1 style="line-height:100px">LEU LOCK</h1> | + | <h1 style="line-height:100px">LEU-LOCK</h1> |
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| − | <p><span class="p">
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| − | <span class="ptitle">How did we choose our project?</span><br><br> Our team split into three small groups in March and each proposed a refined project idea.
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| − | Our other ideas included lysin synthesis to treat acne and the development of a bamboo chassis to combat air pollution.
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| − | After much heated debate and further research into project feasibility, we chose to focus our research on biocontainment.
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| − | We wanted to use a large-scale approach to improve the field of synthetic biology.
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| − | By developing a reliable biocontrol standard, we aim to promote practical, safe implementation of biological devices.
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| − | <br><br><span class="ptitle">Project Description</span><br><br>
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| − | The field of synthetic biology currently struggles with the issue of containment both in laboratory settings and real-world environments.
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| − | This shortcoming prevents the widespread implementation of useful engineered devices and calls for a cellular-based containment system that can operate in an open environment and provide security comparable to physical containment.
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| − | Although several biological methods currently exist for containment, these methods allow some degree of genetic escape through horizontal gene transfer, spontaneous mutagenesis, or utilization of environmentally
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| − | available compounds.
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| − | <span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Mandell, Daniel J., Marc J. Lajoie, Michael T. Mee, Ryo Takeuchi, Gleb Kuznetsov, Julie E. Norville, Christopher J. Gregg, Barry L. Stoddard, and George M. Church.
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| − | "Corrigendum: Biocontainment of Genetically Modified Organisms by Synthetic Protein Design." Nature 527.7577 (2015): 264. Web.
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| − | <a href="http://www.nature.com/nature/journal/v527/n7577/full/nature15536.html" target="_blank">(link)</a></span></span>
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| − | The Virginia iGEM team proposes to use the CRISPR/Cas9 system to redesign leucyl-tRNA synthetase to confer metabolic dependence on modified leucine in <i>Escherichia coli</i>.
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| − | Our goal is to create the foundation for a reliable, standardized, and universally applicable biocontainment system.
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| − | </span></p>
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