Difference between revisions of "Team:Paris Saclay/Notebook/August/5"
(Created page with "{{Team:Paris_Saclay/notebook_header}} = Friday 5<sup>st</sup> August= ==Lab work== ===Visualization=== ==== DNA Extraction of DS-TDcasN- and DS-SPcasN- ==== ''By Laetitia''...") |
(→DNA Extraction of DS-TDcasN- and DS-SPcasN-) |
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The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation. | The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation. | ||
Hence we used: | Hence we used: | ||
| + | |||
-1 ml of resuspension buffer | -1 ml of resuspension buffer | ||
| + | |||
-1 ml lysis buffer | -1 ml lysis buffer | ||
| + | |||
-1 ml precipitation buffer | -1 ml precipitation buffer | ||
The colum was used several times in order to recover the maximum of DNA | The colum was used several times in order to recover the maximum of DNA | ||
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==== DNA Extraction of DS-NMcasN- and pPS_001 ==== | ==== DNA Extraction of DS-NMcasN- and pPS_001 ==== | ||
Revision as of 12:24, 5 August 2016
