Difference between revisions of "Team:Paris Saclay/Notebook/August/5"

(DNA Extraction of DS-TDcasN- and DS-SPcasN-)
(DNA Extraction of DS-TDcasN- and DS-SPcasN-)
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The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation.
 
The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation.
 +
 
Hence we used:
 
Hence we used:
  

Revision as of 12:24, 5 August 2016

Friday 5st August

Lab work

Visualization

DNA Extraction of DS-TDcasN- and DS-SPcasN-

By Laetitia


The extraction was performed using the kit. We followed this protocol.

The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation.

Hence we used:

-1 ml of resuspension buffer

-1 ml lysis buffer

-1 ml precipitation buffer

The colum was used several times in order to recover the maximum of DNA

DNA Extraction of DS-NMcasN- and pPS_001

By Mathilde

The extraction was performed on clone 11 for NM and clone 9 for 1.1 using the kit. We followed this protocol.

By Caroline