Difference between revisions of "Team:Paris Saclay/Notebook/August/11"
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Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | ||
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| + | ====[[Team:Paris_Saclay/Experiments#pPS16_010|pPS16_010]] extraction==== | ||
| + | After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:00, 11 August 2016
