Difference between revisions of "Team:Paris Saclay/Notebook/August/11"
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After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z | After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z | ||
[[File:T--Paris_Saclay--20160811_extraction_FRB_3_7_8.jpeg|400px|thumb|right|Migration of FRB plasmid]] | [[File:T--Paris_Saclay--20160811_extraction_FRB_3_7_8.jpeg|400px|thumb|right|Migration of FRB plasmid]] | ||
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| + | ====DreamTaq PCR of transformed cell with pPS16_002,008,010, 011, 012, 013, 014 and puC 19==== | ||
| + | ''By Léa and Laetitia'' | ||
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| + | PCR DreamTaq was performed on 6 clones of each transformed celles from 10/08/16. | ||
| + | Each clone was plated and put in liquid culture with LB and Ampicillin. | ||
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| + | {{Team:Paris_Saclay/notebook_footer}} | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:13, 11 August 2016
