Difference between revisions of "Team:Paris Saclay/Notebook/August/11"

(Visualization)
(Wednesday 10th August)
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= Wednesday 10<sup>th</sup> August=
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= Thursday 11<sup>th</sup> August=
 
==Lab work==
 
==Lab work==
 
===Visualization===
 
===Visualization===

Revision as of 08:11, 12 August 2016

Thursday 11th August

Lab work

Visualization

Transformation of DH5a cells with pPS16_009

By Léa

Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual protocol.


pPS16_010 extraction

By Alice

After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following this protocol. After extraction, plasmids were migrated on a gel.z

Migration of FRB plasmid

DreamTaq PCR of transformed cell with pPS16_002,008,010, 011, 012, 013, 014 and puC 19

By Léa and Laetitia

PCR DreamTaq was performed on 6 clones of each transformed celles from 10/08/16. Each clone was plated and put in liquid culture with LB and Ampicillin.