Thursday 11^th August

Lab work

Visualization

Transformation of DH5a cells with pPS16_009

By Léa

Dh5a cells were transformed with pPS16_009, or psB1c3 (control), or not transformed (control)using the usual protocol.

pPS16_010 extraction

By Alice

After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following this protocol. After extraction, plasmids were migrated on a gel.z

Migration of FRB plasmid

DreamTaq PCR of transformed cell with pPS16_002,008,010, 011, 012, 013, 014 and puC 19

By Léa and Laetitia

PCR DreamTaq was performed on 6 clones of each transformed celles from 10/08/16. Each clone was plated and put in liquid culture with LB and Ampicillin.