Tuesday 16^th August

Lab work

Visualization

2.1-2.2 and 3.1-3.2 ligation

By Charlène

8µL of 2.1 purify PCR products, 8µL of 2.2 purify PCR products, 2µL of ligase T4 Buffer and 2L of ligase T4 were mixed. 8µL of 3.1 purify PCR products, 8µL of 3.2 purify PCR products, 2µL of ligase T4 Buffer and 2µL of ligase T4 were mixed. They were incubated for 1h at RT.

Q5 PCR on the ligation products and pPS16_008 clones 1 and 2

By Charlène

The PCR was carried out with a new protocol :

The specific primers for each parts were using. The products were put to migrated on a 0.8%agarose gel with BET.