Difference between revisions of "Team:Paris Saclay/Notebook/August/24"

(→‎Visualization)
(→‎pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction)
Line 24: Line 24:
  
  
 +
Nano drop :
  
 +
{| class="wikitable"
 +
|-
 +
!
 +
!size (ng/µL)
 +
!260/230
 +
!260/280
 +
|-
 +
|1.2.14
 +
|47.81
 +
|1.64
 +
|1.84
 +
|-
 +
|1.2.8
 +
|834.5
 +
|2.40
 +
|1.99
 +
|-
 +
|KCl
 +
|250mM
 +
|-
 +
|SgRna Nm 12
 +
|124.21
 +
|2.00
 +
|1.89
 +
|}
  
  

Revision as of 12:56, 24 August 2016

Wednesday 24th August

Lab work

Visualization

Migration gel of Gibson (fragment 3 + fragment 4) and GFP in PSB1C3

By Terrence


Clone 6 and 8 seems correct for the Gibson.


Clone 2-4-7-11 are correct.


pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction

By Terrence

The extraction was carried out following the usual protocol.


Result of the extraction


Nano drop :

size (ng/µL) 260/230 260/280
1.2.14 47.81 1.64 1.84
1.2.8 834.5 2.40 1.99
KCl 250mM
SgRna Nm 12 124.21 2.00 1.89







Université Paris Sud
91405 Orsay, France
Copyright © 2016 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.