Difference between revisions of "Team:Paris Saclay/Notebook/August/24"

(pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction)
(pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction)
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|2.40
 
|2.40
 
|1.99
 
|1.99
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|KCl
 
|250mM
 
 
|-
 
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|SgRna Nm 12
 
|SgRna Nm 12

Revision as of 12:57, 24 August 2016

Wednesday 24th August

Lab work

Visualization

Migration gel of Gibson (fragment 3 + fragment 4) and GFP in PSB1C3

By Terrence


Clone 6 and 8 seems correct for the Gibson.


Clone 2-4-7-11 are correct.


pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction

By Terrence

The extraction was carried out following the usual protocol.


Result of the extraction


Nano drop :

size (ng/µL) 260/230 260/280
1.2.14 47.81 1.64 1.84
1.2.8 834.5 2.40 1.99
SgRna Nm 12 124.21 2.00 1.89