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− Then we transformed 2µL of ligation product into 50 µL of competent cells, following the usual protocol
+ Then we transformed 2µL of ligation product into 50 µL of competent cells, following the [[Team:Paris_Saclay/Experiments#Heat_shock_competent_cells| usual protocol]].
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Revision as of 14:21, 24 August 2016
Wednesday 24th August Lab work Visualization Migration gel of Gibson (fragment 3 + fragment 4) and GFP in PSB1C3 By Terrence
Clone 6 and 8 seems correct for the Gibson.
Clone 2-4-7-11 are correct.
pPS16_002 (1.2) clones 8 and 14 and pPS16_014(SgRna Nm) clone 12 Extraction By Terrence
The extraction was carried out following the usual protocol .
Nano drop :
size (ng/µL)
260/230
260/280
1.2.14
47.81
1.64
1.84
1.2.8
834.5
2.40
1.99
SgRna Nm 12
124.21
2.00
1.89
Transformation of pPS16_010 (FRB) in pJET and pPS16_014 (SgRnaNm) in pJET By Terrence
Component
Volume (µL)
Reaction Buffer
10
PCR Product (FRB or Nm)
1
pJET1.2/blunt
1
water, nuclease free
7
T4 DNA Ligase
1
Then we transformed 2µL of ligation product into 50 µL of competent cells, following the usual protocol .
