Difference between revisions of "Team:Paris Saclay/Notebook/August/23"

(Created page with "{{Team:Paris_Saclay/notebook_header}} = Tuesday 23<sup>th</sup> August= ==Lab work== ===Visualization=== ====Cell Cultures==== ''By Léa, Naiane and Mathilde'' 100µL ofDH5a...")
 
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Interlab study plates from the 22/08/2016 were used to inoculate two tubes containg 5mL of liquid LB medium (chloramphenicol) per construction.
 
Interlab study plates from the 22/08/2016 were used to inoculate two tubes containg 5mL of liquid LB medium (chloramphenicol) per construction.
  
DH5a colonies containing puc19 vector cloned with sgRNA NM (clone 5 and 12) or 1.2 (clones 8 and 14) were inoculated into 4mL of liquid LB medium.
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DH5a colonies containing pUC19 vector cloned with sgRNA NM (clone 5 and 12) or 1.2 (clones 8 and 14) were inoculated into 4mL of liquid LB medium.
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 10:15, 25 August 2016

Tuesday 23th August

Lab work

Visualization

Cell Cultures

By Léa, Naiane and Mathilde

100µL ofDH5a precultures containing dCas9 Nm plasmids (clone 1 and 2) were used to inoculate 200mL of liquid LB medium containing spectinomycin.

Interlab study plates from the 22/08/2016 were used to inoculate two tubes containg 5mL of liquid LB medium (chloramphenicol) per construction.

DH5a colonies containing pUC19 vector cloned with sgRNA NM (clone 5 and 12) or 1.2 (clones 8 and 14) were inoculated into 4mL of liquid LB medium.