Difference between revisions of "Team:Paris Saclay/Notebook/August/24"
(→Gel Electrophoresis of Gibson (fragment 3 + fragment 4) and GFP in PSB1C3) |
(→Transformation of pPS16_010 (FRB) in pJET and pPS16_014 (SgRnaNm) in pJET) |
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| − | ====Transformation of | + | ====Transformation of gblockFRB in pJET and gblock Sg_RNA_NM in pJET==== |
''By Terrence'' | ''By Terrence'' | ||
| Line 76: | Line 76: | ||
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| − | Then we transformed 2µL of ligation product into 50 µL of competent cells, following the [[Team:Paris_Saclay/Experiments#Heat_shock_competent_cells|usual protocol]]. | + | Then we transformed 2µL of ligation product into 50 µL of DH5a competent cells, following the [[Team:Paris_Saclay/Experiments#Heat_shock_competent_cells|usual protocol]]. |
| − | Transformation products were plated | + | Transformation products were plated with three different volume : 50µL , 150µl and 350µL. 350µL only for the control. |
====Plasmid DNA extraction (Midi Prep)==== | ====Plasmid DNA extraction (Midi Prep)==== | ||
Revision as of 10:18, 25 August 2016
