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+ This question is essential because the distance between the dCas9 may cause major problem: if this system doesn’t work we may not see the effect of the other system. In fact, the 3D structure of our linker may interact with the dCas9 or the GFP because of steric hindrance.
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Revision as of 15:15, 8 September 2016
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Introduction One of the goal of our project is to visualize the system “bring DNA closer” with the tri-partite GFP.
In a first place, we decide to focus the modeling part on the question “what is the optimal distance between the two dCas9 for fluorescence?”
Our system is divide in 4 parts:
Two dCas9
Two linker
Tri-partite GFP This question is essential because the distance between the dCas9 may cause major problem: if this system doesn’t work we may not see the effect of the other system. In fact, the 3D structure of our linker may interact with the dCas9 or the GFP because of steric hindrance.
Modeling
Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.
Inspiration
Here are a few examples from previous teams:
