Line 94: | Line 94: | ||
<h2>Lab work</h2> | <h2>Lab work</h2> | ||
<p>During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.</p> | <p>During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.</p> | ||
− | + | <div id="safety" class="container-fluid text-center"> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | <div id=" | + | |
<h2>SERVICES</h2> | <h2>SERVICES</h2> | ||
<h4>What we offer</h4> | <h4>What we offer</h4> | ||
Line 112: | Line 101: | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-off logo-small"></span> | <span class="glyphicon glyphicon-off logo-small"></span> | ||
− | <h4> | + | <h4>Use of lab coat</h4> |
− | + | ||
</div> | </div> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-heart logo-small"></span> | <span class="glyphicon glyphicon-heart logo-small"></span> | ||
− | <h4> | + | <h4>Use of nitrile gloves</h4> |
− | + | ||
</div> | </div> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-lock logo-small"></span> | <span class="glyphicon glyphicon-lock logo-small"></span> | ||
<h4>JOB DONE</h4> | <h4>JOB DONE</h4> | ||
− | <p> | + | <p>Use of safety goggles</p> |
</div> | </div> | ||
</div> | </div> | ||
Line 130: | Line 117: | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-leaf logo-small"></span> | <span class="glyphicon glyphicon-leaf logo-small"></span> | ||
− | + | <p>Management of bacteria in biosafety cabinet</p> | |
− | <p> | + | |
</div> | </div> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-certificate logo-small"></span> | <span class="glyphicon glyphicon-certificate logo-small"></span> | ||
− | <h4> | + | <h4>Usage of volatile chemicals in fume hood</h4> |
− | + | ||
</div> | </div> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-wrench logo-small"></span> | <span class="glyphicon glyphicon-wrench logo-small"></span> | ||
− | <h4 | + | <h4>Cleaning and Sterilization of the Lab</h4> |
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
Line 148: | Line 132: | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-leaf logo-small"></span> | <span class="glyphicon glyphicon-leaf logo-small"></span> | ||
− | <h4> | + | <h4>Correct disposal of bio-hazardous residues</h4> |
− | + | ||
</div> | </div> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<span class="glyphicon glyphicon-certificate logo-small"></span> | <span class="glyphicon glyphicon-certificate logo-small"></span> | ||
− | <h4> | + | <h4>Separate areas for work with bacteria, DNA and electrophoresis</h4> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
Line 167: | Line 144: | ||
<p>In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.</p> | <p>In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.</p> | ||
</main> | </main> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</body> | </body> | ||
</html> | </html> | ||
{{:Team:TEC-Costa_Rica/Template:Scripts}} | {{:Team:TEC-Costa_Rica/Template:Scripts}} | ||
{{:Team:TEC-Costa_Rica/Template:Footer}} | {{:Team:TEC-Costa_Rica/Template:Footer}} |
Revision as of 01:02, 23 September 2016
Safety
Our project is related to the detection of a human disease via a biomarker molecule found in the urine of patients with prostate cancer. Since local regulation does not allow us to work with human samples, we designed our project in a way that it can work as a proof-of-concept of a detection system and pose no risk to human health.
Lab work
During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.
SERVICES
What we offer
Project safety
In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.