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In order to answer our question, we choose to create a new technology to bring two different DNA regions closer. As a results, we have designed a linking tool and a visualization tool.

Here, we will expose you our experimental strategy, as well as, the biobricks we have designed to do so.

Linking tool construction

Visualization tool construction

Characterization strategy

Tripartite Split-GFP and FRB/FKBP12 dimerization systems

Assessment of the minimal distance to have fluorescence

Assessment of the DNA regions brought closer

Gene expression tests