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Overview

Recent observations lead to the idea that genes, not in the same operon but spatially close, are highly co-transcribed, even in the absence of regulatory factors at their promoter regions.

The iGEM Paris-Saclay project aims to study the effects of DNA topology on gene expression in E.coli by answering to this question: Does bringing a strong promoter closer to a weak promoter influence the expression level of genes located downstream?

We have designed a new tool based on CRISPR/Cas9 system to bring two specific DNA regions closer. This system is composed of two different dCas9 fused with each part of FRB / FKBP12 dimerization system. Each dCas9 will target a specific DNA sequence, one on the chromosome and one on a plasmid, whereas dimerization system will promote the joining of the two dCas9 when rapalog is added.

In order to assess whether or not this system works, we have also designed a new tool to visualize the interaction between both dCas9. This tool is composed of a split GFP attached to two dCas9. These two small GFP tags will interact with the complementary GFP detector only if the two dCas9 are closed enough to interact.

If we obtain a highest expression level of the weak promoter with our two tools, it could lead to several useful applications. For example, we would be able to use this tool to enhance gene expression of any endogenous genes due to CRISPR/Cas9 specificity. Indeed, it would be possible to design specific sgRNA but user should be aware about off-target activity of the CRISPR/Cas9 system.

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Backgroung

Introduction

Transcription is the first step of the Central dogma of molecular biology. It is the process in which an RNA molecule is created based on the sequence of a DNA template [Fig. 1]. In this case the template is a gene, a region of DNA who’s RNAs can be translated into proteins, which perform different functions within the cell. Because the environment of bacteria is constantly changing, cells have to adapt. A part of adaptation consists in using different proteins present at different times in the cell. These changes can be the result of transcriptional regulation.

Numerous molecules and factors are involved in the mechanism of transcription and can be involved in its regulation. Among these, RNA polymerase is the protein that performs transcription [Fig. 1]. Transcription factors are proteins that enhance or prevent transcription; other factors such as small molecules, DNA sequence properties, and chromosome structure also play a role even though these mechanisms are diverse and often poorly understood (Browning 2002) [Fig. 2]. Sequences called promoters are small DNA sequences upstream of genes. RNA polymerase binds to them before starting transcription.

RNA polymerase is composed of several parts, each of them called a “subunit”. In bacteria, it’s one of these subunits that binds first to the promoter. It is called “σ-factor” [Fig. 3]. But other factors can be necessary to begin transcription or to prevent it, especially when the promoter isn’t strong enough to keep the σ-factor attached to it. All of the proteins that can bind to DNA are called DNA binding protein. The binding of a multisubunit RNA polymerase or general transcription factors to a specialized transcription promoter DNA sequence is an essential step in initiating DNA transcription in all organisms (Friedman 2013).

DNA binding proteins

These DNA binding proteins are able to bind to DNA with different strengths also called affinities. Some proteins bind DNA with the same affinity regardless of the DNA sequence, others can only be recruited by specific sequences. Four mechanisms by which a DNA binding protein finds its target sequence are known: sliding, when the protein slides along DNA [Fig. 4a]; hopping, when the protein unbinds a DNA segment and binds to another one [Fig. 4b]; intersegment transfer, when the protein binds two different segments of DNA before releasing one; and looping, which consists on the binding of two DNA segments leading to a loop (Bustamante 1999, Bondos 2015) [Fig. 4c,d]. Among these, only the one-dimensional sliding is independent of spatial organization in the chromosome, that’s why the structural organization of genomes is very important for transcriptional regulation.

Competition for binding within a cell

These four mechanisms allow for dynamic binding of proteins to their target sequences, despite the fact that these proteins are not present in large concentrations. For example, the number of genes in Escherichia coli is about 5 000 and the number of active RNA polymerases is about 200 per cell, of which 140 only transcribe 2% of the genes leaving 60 polymerases for the other 4995 genes. Thus, there is tight competition between genes to get transcribed: competition between the same σ-factor for several promoters, competition between σ-factors for the rest of the RNA polymerase or competition between a σ-factor and other DNA binding proteins. These competitions are fundamental in the mechanism of transcriptional regulation. They are often allowed or prevented by remodeling of the chromosome topology (Haugen 2008) [Fig. 4].

That’s why, the link between genome topology and gene regulation is a major research topic in the subject of transcription. Although the mechanism of global gene regulation by changes in DNA organization are well established, the fine tuning of transcription is particularly hard to study and not very well understood. Considering this gap in knowledge, new studies have to be without a priori in order to determine if fine tuning of the DNA topology takes place during transcriptional regulation and if so, how does such regulation work.

Transcription initiation

Regulation can occur at every step on the pathway to gene expression, but transcription initiation is probably the most frequently regulated step (Haugen 2008). It is the result of 1) the recognition of a promoter by a σ-factor, 2) the recruitment of the other parts of the RNA polymerase, 3) the formation of an open complex, thus the unwinding of DNA from either side of the promoter 4) the beginning of RNA synthesis and finally 5) the release of the σ-factor (Browning 2002) [Fig. 6]. The first step, in which the σ-factor binds to the promoter, can limit the transcription rate. Moreover one σ-factor can lead to the beginning of transcription of a gene, be released and lead to the beginning of the transcription of an other one. That’s why we think that spatially close promoters can act on each others, for example by doubling the number of sequences which can recruit a σ-factor in a certain area. A strong promoter could recruit a σ-factor and after its release, this σ-factor would have a better probability of being recruited to a weaker promoter. Conversely, a strong promoter could have a better chance of recruiting a σ-factor leading to “starving” of the weaker promoters within the same area.

Question

To understand transcriptional regulation, we have to study chromosome topology in 4-dimensions (3D + time) and its effect on the recruitment of DNA binding proteins. In this project, we try to answer to a fundamental question: Are proximal promoters influencing each other’s transcriptional rates? [Fig. 5].