Difference between revisions of "Team:SCAU-China/Collaborations"

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height:780px;
 
height:780px;
 
position:relative;
 
position:relative;
background:url(./images/header-img.jpg) no-repeat center bottom;
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background:url(https://static.igem.org/mediawiki/2016/6/60/Header-img.jpg) no-repeat center bottom;
 
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#header .menu{
 
#header .menu{
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margin:0 auto;
 
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cursor:pointer;
background:url(../images/triangle.png) no-repeat;
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#content .vecter .nav{
 
#content .vecter .nav{
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       <li><a href="#modeling">Modeling</a></li>
 
       <li><a href="#modeling">Modeling</a></li>
 
       </ul>
 
       </ul>
       <div class="img"><img src="images/img_1.jpg"></div>
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       <div class="img"><img src="https://static.igem.org/mediawiki/2016/a/a6/Img_1.jpg"></div>
 
       <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
 
       <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
 
Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
 
Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
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       </ul>
 
       </ul>
 
       <div class="img">
 
       <div class="img">
       <p><img src="images/img_2.jpg"></p>
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       <p><img src="https://static.igem.org/mediawiki/2016/0/0f/Img_2.jpg"></p>
       <p><img src="images/img_3.jpg"></p>
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       <p><img src="https://static.igem.org/mediawiki/2016/9/94/Img_3.jpg"></p>
       <p><img src="images/img_4.jpg"></p>
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       <p><img src="https://static.igem.org/mediawiki/2016/8/83/Img_4.jpg"></p>
 
       </div>       
 
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       </ul>
 
       </ul>
 
       <div class="img">
 
       <div class="img">
       <p><img src="images/img_5.jpg"></p>
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       <p><img src="https://static.igem.org/mediawiki/2016/3/35/Img_5.jpg"></p>
       <p><img src="images/img_6.jpg"></p>
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       <p><img src="https://static.igem.org/mediawiki/2016/9/97/Img_6.jpg"></p>
 
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       </ul>
 
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       <div class="img">
 
       <div class="img">
       <p><img src="images/image014.jpg">&nbsp;&nbsp;<img src="images/image016.png">&nbsp;&nbsp;<img src="images/image015.png"></p><br>
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       <p><img src="https://static.igem.org/mediawiki/2016/0/07/Image014.jpg">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/b/ba/Image016.png">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/5/5b/Image015.png"></p><br>
       <p><img src="images/image020.jpg">&nbsp;&nbsp;<img src="images/image022.png">&nbsp;&nbsp;<img src="images/image018.png"></p>
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       <p><img src="https://static.igem.org/mediawiki/2016/7/77/Image020.jpg">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/2/28/Image022.png">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/d/d3/Image018.png"></p>
 
       </div>       
 
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       <li><a href="#modeling">Modeling</a></li>
 
       <li><a href="#modeling">Modeling</a></li>
 
       </ul>
 
       </ul>
       <div class="img"><img src="images/image024.png"></div>
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       <div class="img"><img src="https://static.igem.org/mediawiki/2016/0/0d/Image024.png"></div>
 
       <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
 
       <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
 
HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
 
HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
 
Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div>   
 
Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div>   
<div class="img"><img src="images/image026.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/8/8d/Image026.png"></div>
<div class="img"><img src="images/image028.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/f/fe/Image028.png"></div>
<div class="img"><img src="images/image030.png"></div>     
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/8/82/Image030.png"></div>     
 
     </div>
 
     </div>
 
      
 
      
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       <li><a href="#modeling" class="on">Modeling</a></li>
 
       <li><a href="#modeling" class="on">Modeling</a></li>
 
       </ul>
 
       </ul>
       <div class="img"><img src="images/image032.png"></div>
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       <div class="img"><img src="https://static.igem.org/mediawiki/2016/9/94/Image032.png"></div>
 
       <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div>   
 
       <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div>   
 
     </div>
 
     </div>

Revision as of 12:22, 4 October 2016

Fig.1 Not I digestion of constructs cotaining different numbers of genes. Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
Fig.n HPLC analysis diagram of standard samples of astaxanthin(a); HPLC analysis diagram of samples extract from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);

Compared with the standard samples with HPLC analysis diagram, you can see the sample extract from rice and standard samples have the same peak at the same time, show that the transgenic rice seed which carry the four genes expressed astaxanthin. Results indicate that we are successful synthesize astaxanthin by rice endosperm synthetic .
Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .