</a>We have to know that arboviruses don’t represent a single family of viruses but several viruses from different families: Flaviviridae (Dengue virus DENV, Zika virus ZIKV[1], Yellow Fever virus YFV, West Nile virus WNV, etc), Togaviridae (Chikungunya virus CHIKV, Ross River virus RRV, etc), Bunyaviridae (Rift Valley virus RVV, etc), that’s why specific treatments or vaccines need to be adapted for each family (See <a href="https://2016.igem.org/Team:Pasteur_Paris/Context_ID">IDENTITY CARDS</a> section)[2][3]. </br></br>

By infecting more than 400 million people per year, dengue virus (DENV) is the most important arbovirus. In recent decades, the global incidence of dengue has risen sharply and the transmission has increased in urban and suburban areas. More recently, Zika virus has been on the news : outbreaks occurred in French Polynesia (2013) and Brazil (2016), causing serious neurological complications such as Guillain-Barré syndrome and microcephaly.  </br></br>

One of the particularity of the arboviruses is that they infect two kinds of host : a mammalian host that ensure the maintainance and the amplification of the virus, and an arthropod vector (mosquitoes) that ensure its dissemination. In fact, arboviruses originally circulate in a sylvatic cycle in which the virus is transmitted between non-human primates by zoophilic mosquitoes. During its repeated intrusions into forests (hunting, deforestation…), men are accidentally contaminated by contact with anthropo-zoophilic vectors. Then, anthropophilic vectors ensure the dissemination of arboviruses between humans in urban areas, during the urban cycle. </br></br>

First, a female lays between 50-300 eggs where standing water is present: a small amount of water is  sufficient and breeding sites can be natural (flooded grasslands, waste water) or artificial (tires,  bowls, cups, fountains, tires, barrels, vases). These eggs stick to container walls like glue, and hatch between 3 to 7 days after egg-laying according to environmental conditions. In case of not suitable conditions, some eggs can resist to desiccation for several years or enter in diapause. </br></br>

Second, when water covers completely the eggs, larvae emerge. Often called “wigglers”, larvae are active in water, where respiration occurs thanks to a siphon on the 8th segment of the abdomen of the larvae. Hung upside-down, larvae eat organic matter such as algae, microscopic organisms in order to ensure its development to the third stage: the pupae. </br></br>

Instead of larval stage, pupae are not active and do not feed. A pair of respiratory trumpets allows pupae to live 2-3 days. </br></br>

Fortunately, all mosquitoes are not able to transmit arboviruses! The competence of a mosquito is the ability of a vector to be infected by and to transmit a pathogen in natural conditions. For example, competent vectors of malaria are Anopheles mosquitoes. Competent vectors for DENV, CHIKV, ZIKV, YFV, RRV, etc are Aedes aegypti and Aedes albopictus mosquitoes. </br></br>

Since only yellow fever is prevented by vaccination, the single efficient method to fight against arboviruses is vector control. Several methods exist but the most common is insecticide spraying (particularly pyrethroids and organophosphates). Unfortunately, mosquitoes become more and more resistant and more and more classes of insecticides are developed, increasing multiresistances and high environmental impact. Moreover, since we are unable to maintain effective control of mosquito populations, the fact that resistance contributes to the re-emergence of arboviruses cannot be excluded[5][6]. That’s why insecticide resistance is now regarded by the WHO as a major obstacle to the control of diseases transmitted by mosquitoes. Many organizations are created and act together. For example, in accordance with the Research and Training Program on Tropical Diseases (TDR), supported by WHO, the Department of Neglected Tropical Diseases (NTDs) and the WIN network met for the first time. WIN network is comprised of fifteen international institutions recognized for research on vectors to counter the resistance to insecticides globally. Additionally, the IRD, CNRS and the Institut Pasteur of French Guiana are highly mobilized at the French level. Concerning France, insecticides are sprayed when human infected cases are identified and where competent mosquitoes are present. Considering asymptomatic carriers, symptomatic carriers who think having flu, and physicians who don’t report identified cases, the majority of disease importations are not detected[7][8]. Moreover, when identified cases are reported, insecticide spraying occursthere, ignoring the possibility of mobility of infected people or infected mosquitoes. It seems obvious that it could be better to anticipate disease importations and outbreaks occurrences by acting before infected cases identification happens[9][10]. Finally, competent vectors repartition all over the world is well known but infected vectors repartition is not. By mapping the infected vectors’ repartition, we could perform a more rational use of insecticides and focus our efforts on concerned regions[11][12]. </br></br>

In this context, our Mos(kit)O project consists in the development of the infected mosquitoes mapping, this indispensable tool that vector control is lacking. Let us present to you our Mos(kit)O project in <a href="https://2016.igem.org/Team:Pasteur_Paris/Overview">OVERVIEW</a> section!