Difference between revisions of "Team:Paris Saclay/Notebook/October/6"
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After extraction, 5 µL of each plasmids and 20 µL of DNA ladder were placed in wells and migrated at 100V during 30 min in order to assess their concentration. | After extraction, 5 µL of each plasmids and 20 µL of DNA ladder were placed in wells and migrated at 100V during 30 min in order to assess their concentration. | ||
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| + | GEL | ||
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| + | ====Transformation of DH5a cells with and FKBP - GFP 10 (pPS16_018) and FRB - GFP 11 (pPS16_019)==== | ||
| + | ''By Maxence & Victor'' | ||
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| + | As we were not able to obtain good plasmid concentration after culture of glycerol stocks and plasmid extraction, transformations were done. Dh5a cells were transformed with pSB1C3 containing FKBP - GFP 10 (pPS16_018) and FRB - GFP (pPS16_019) using the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | ||
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| + | ===BioBrick X characterization=== | ||
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| + | ====Plasmids extraction of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4 and FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6==== | ||
| + | ''By Maxence & Victor'' | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 10:19, 7 October 2016
