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Revision as of 06:21, 9 October 2016

Attributions in AI-2 Controller Construction
Fankang Meng, Xinyu Ma, Yujie Zhao, Jingzhe Dong:
AI-2 Controller Gene Circuits Construction;
AI-2 Response Device Construction;
AI-2 Controller Biosafety Construction;
Xiao Liu, Xinhao Song, Liangti Dai:
AI-2 Controller Characterization;
Parts Construction;
Interlab Study;
Zhuoer Dong:
Mathematical Models; Wiki Construction;
Xianglin Zhao:
Human Practice; Art Design;
Attributions in details
Project: the idea
We came to the "AI-2 Controller" project after many brainstorming meetings including all the team. It would be impossible to define authors here: we all contributed to the design of the project.
AI-2 Controller Construction:
All the experiments including AI-2 controller gene circuits construction, AI-2 response device construction and AI-2 controller biosafety construction was designed and conducted by Fankang Meng, Xinyu Ma, Yujie Zhao, Jingzhe Dong.
This part of construction includes using homologous recombination technology to construct pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK, AI-2 Response Device A and B. What's more, these four people also take advantage of mf-lon protease to create safer engineered bacteria.
AI-2 Controller Characterization:
All the experiments in AI-2 controller characterization was designed and conducted by Fankang Meng, Xiao Liu and Xinhao Song. This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
Model:
This part of work was designed and conducted by Zhuoer Dong, Meng Fankang.
Parts Construction:
Xinhao Song, Liu Xiao, Liangti Dai and Yuxiu Xiong performed all the work for parts standardization.
Interlab Study:
Liu Xiao, Xinhao Song, Liangti Dai and Yuxiu Xiong performed all the work for interlab study.
Human Practices.
Xinglin Zhao, Liangti Dai, Zhuoer Dong and Ruilin Tian organized the "Synfuture" Exposition. Also they organized the iGEM Meeting with iGEM team TJUSLS_China. What's more, they also interviewed many famous scholars to get suggestions which greatly improved our project.
Wiki Construction
This part of work was designed and conducted by Zhuoer Dong, Xianglin zhao.
Supervision
THE INSTRUCTORS were closely involved keeping the message clear and the team on track to reaching the goals.
Dr. Yufen Quan as our instructor keeps the great mood in the lab and his accurate questions leads everyone to the result without any need for explanation.
Dr. Yulei Dang provided tremendous organizational support for our team throughout the duration.
Dr. Jiwei Mao & Dr. Xiang Wang gave us helpful advice on AI-2 Controller Protein level experiments.
Acknowledgements
We would like to thank the The Ministry Key Laboratory of Molecular Microbiology and Technology and School of Boling for helping us construct the new iGEM laboratory. Namely we would also like to thank Prof. Weihui Wu, Dr. Honggang Wang for letting us use the microplate and fluorescence microscope when we characterized the AI-2 Responser. We would like to also thank Prof. Lingyi Chen and Prof. Zhihui Cheng for sharing their experience with circuits construction and for giving us helpful advice on how to improve our experiments to get better data.
Thanks to all our colleagues at the College of Life Sciences for their support during our research. Special thanks belongs to Prof. Cunjiang Song for opening up his Introduction to biotechnology course for an hour allowing us to present our project to freshmen in the College of Life sciences.
Finally, we want to thank teams listed below for helping us improve our project on data, model, human practice, etc.
  • iGEM Team NAU_China;
  • iGEM Team SLSTJU;
  • iGEM Team Tianjin;