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+ | =Thursday 7<sup>th</sup> July= | ||
+ | ==Lab work== | ||
+ | ===Visualization=== | ||
+ | ====PCR products migation==== | ||
+ | ''By Caroline and Léa'' | ||
+ | gBlocks screened the [[Team:Paris_Saclay/Notebook/July/6#Visualization|06/07/2016]] were put on migration. | ||
+ | The usual [[Team:Paris_Saclay/Experiments#DNA_electrophoresis_on_agarose_gel|protocol]] was followed. | ||
+ | 20µL of each PCR product was added to 4µL of purple loading dye 6X. | ||
− | + | PHOTO GEL pas dans le cahier ! | |
− | + | ====Cultures results==== | |
− | + | There was no blue colony observed on xGal/IPTG + ampicillin mediums for transformed [[Team:Paris_Saclay/Notebook/July/6#VisualizationVpPS16_004, pPS16_007 and the pPS16_007 clone 6 gBlocks]]. | |
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− | + | {{Team:Paris_Saclay/notebook_footer}} | |
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Revision as of 09:06, 19 July 2016