Difference between revisions of "Team:Paris Saclay/Parts"

(Existing parts used for the project)
(Existing parts used for the project)
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|35
 
|35
 
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|[http://parts.igem.org/Part:BBa_B0030/ BBa_B0030]
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|[http://parts.igem.org/Part:BBa_B0030 BBa_B0030]
 
|Basic
 
|Basic
 
|RBS.1 (strong) - modified from R. Weiss
 
|RBS.1 (strong) - modified from R. Weiss
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|15
 
|15
 
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|[http://parts.igem.org/Part:BBa_B0015/ BBa_B0015]
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|[http://parts.igem.org/Part:BBa_B0015 BBa_B0015]
 
|Basic
 
|Basic
 
|Double terminator (B0010-B0012) (rrnB T1 - T7Te)
 
|Double terminator (B0010-B0012) (rrnB T1 - T7Te)

Revision as of 19:09, 14 October 2016

Parts

Existing parts used for the project

In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:


Name Type Description Designer Length (bp)
[http://parts.igem.org/Part:BBa_J23119 BBa_J23119] Basic Constitutive promoter family member iGEM2006_Berkeley 35
[http://parts.igem.org/Part:BBa_B0030 BBa_B0030] Basic RBS.1 (strong) - modified from R. Weiss Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003 15
[http://parts.igem.org/Part:BBa_B0015 BBa_B0015] Basic Double terminator (B0010-B0012) (rrnB T1 - T7Te) Reshma Shetty, 2003 129

Parts designed for the project

In order to achieve our project; we designed several basic and composite parts:


Name Type Description Length (bp)
[http://parts.igem.org/Part:BBa_K2039003/ BBa_K2039003] Basic Coding sequences of proteins FKBP and GFP10 528
[http://parts.igem.org/Part:BBa_K2039004/ BBa_K2039004] Basic Coding sequences of proteins FRB* and GFP11 498
[http://parts.igem.org/Part:BBa_K2039005/ BBa_K2039005] Basic Coding sequences of protein GFP 1.9 633
[http://parts.igem.org/Part:BBa_K2039000/ BBa_K2039000] Composite Part expressing the dimerization protein FKBP and GFP10 731
[http://parts.igem.org/Part:BBa_K2039001/ BBa_K2039001] Composite Part expressing the dimerization protein FRB* and GFP11 701
[http://parts.igem.org/Part:BBa_K2039006/ BBa_K2039006] Composite Part expressing the protein GFP 1.9 836
[http://parts.igem.org/Part:BBa_K2039002/ BBa_K2039002] Composite Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 1440
[http://parts.igem.org/Part:BBa_K2039007/ BBa_K2039007] Composite Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9) 2284