Difference between revisions of "Team:SDU-Denmark/Notebook"

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<h4>Week 26</h4>
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<button class="accordion">Received gBlock, K201843, and resuspend. Date: 16.06.27 - Silk</button>
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<p>The brick was ordered from IDT and received: The received gblock had a weight of 200 ng. and was resuspended in dH2O.
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5 µl diluted gblock and 95 µl ddH2O to make a 20X dilution.
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What we have done with the BioBrick K2018043 can be read in this protocol: <span style="color: #ff0000;">Link to protocol (</span><span style="font-weight: 400;">K2018043 - Cloning Basic part into iGEM standard plasmid psB1C3</span></span> <p>
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<button class="accordion">Week 11</button>
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  <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.</p>
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<h4>Week 28</h4>
 
<h4>Week 28</h4>
 
<button class="accordion">Verification of K2018011 in standard iGEM plasmid (pSB1C3). Date: 16.07.14</button>
 
<button class="accordion">Verification of K2018011 in standard iGEM plasmid (pSB1C3). Date: 16.07.14</button>

Revision as of 21:19, 14 October 2016

Notebook

Week 26

The brick was ordered from IDT and received: The received gblock had a weight of 200 ng. and was resuspended in dH2O. 5 µl diluted gblock and 95 µl ddH2O to make a 20X dilution. What we have done with the BioBrick K2018043 can be read in this protocol: Link to protocol (K2018043 - Cloning Basic part into iGEM standard plasmid psB1C3

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Week 28

The brick containing the gene K2018011 was digested with PstI and XbaI overnight by using SOP0017_v01.

After digesting, the sample was ligated by following SOP0015_v01 with the standard iGEM plasmid, pSB1C3, overnight, and transformed into E.coli Top10.

cPCR program used:

Segment Step Temperature Duration
1 Initial denaturation 98 °C 2 min
2 34 cycles 98 °C 10 sec
    72 °C 15 sec
    72 °C >15 sec
3 Final extension 72 °C 5 min
4 Keep the sample cold 12 °C HOLD

After a colony PCR, SOP0021_v01, was run and verified on a gel, the following result was given: Our part is 259 bp and primer overhangs are 150 X 2 bp, which is, in total, 559 bp: A gel electrophoresis from cPCR showed a band around 600 bp, so we assumed that we had the correct part inserted into the iGEM standard plasmid.

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.