Monday 27^th June

Interlab study

Cell culture

By Lea

Transformed bacteria frozen on 24/06/2016 were put into 3mL of LB medium containing 30µg/mL chloramphenicol and incubated overnight at 37°C, 180rpm.

Visualization

Visualization

By Caroline, Charlène and Naïane

gBlocks from IDT were received on the 24/06/2016.

pUC19 plasmid was digested using HincII restriction enzyme.

The mix was incubated for 1 hour at 37°C. A migration on electrophoresis gel (0.8% of agarose)was done for 30s.

A DNA-ladder was used to visualize the DNA fragments size.

Bringing DNA closer

Cell culture

By Alice and Lea

4 plasmids containing NM Cas9 (DS-NMcas), SP dCas9 (DS-SPcasN-), ST1 dCas9 (DS-ST1casN-) and Td dCas9 (DS-TDcasN-) were ordered to Addgene and received on 24/06/2016.

Plasmids were delivered into tubes containing LB agar medium and bacteria colony containing plasmids. Bacteria were put into 15mL of LB medium containing 50µg/mL of spectinomycin and incubated overnight at 37°C, 180 rpm.

BioBrick characterization

By Alice and Lea

BioBrick [http://parts.igem.org/Part:BBa_K1372001 K1372001] from iGEM team Paris Saclay 2014 was chosen to be characterized.

10µL of DH5α|K1372001 cells were put into 3mL of LB medium containing 30µg/mL of chloramphenicol and incubated overnight at 37°C, 180 rpm.