Difference between revisions of "Team:Rice"

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<h1>project description</h1>
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  <p>The photoacoustic effect describes the conversion of electromagnetic energy to mechanical energy, namely, that an object absorbing non-ionizing laser pulses experiences local thermal expansions, and vibrates with frequencies in the ultrasonic range which may be detected. Imaging based on this effect yields high contrast from the optical component, and high resolution from the acoustic component (1). For biomedical purposes, users of this technique take advantage of endogenous and exogenous contrast agents to obtain physiological information from the biological tissue, endogenous examples including oxy- and deoxy-hemoglobin to determine blood flow speed (2). The bacterial pigment Violacein (Vio) has been reported to be an effective contrast agent under this technique (3). Furthermore, previous iGEM teams have developed and optimized a biosynthesis pathway for this pigment (4, 5).
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This team seeks to build upon, and move forward from, these past investigations and develop a biosensor in E. coli to produce Violacein in the presence of significant concentrations of biomarkers for disease; naturally, the team’s search for potential biomarkers will be for those which may pass through an animal’s or human’s gastrointestinal tract. This team considers the additional design aspect of logic gates to modulate the specificity for our system. Alongside Violacein, this team will experiment with similar genetic circuits using the fluorescent protein iRFP. For the purposes of testing the resultant system(s), this team has made an arrangement with a group at MD Anderson who can introduce our bacteria into mice, and who have photoacoustic imaging equipment to image the bacteria within the gastrointestinal tracts of the mice.
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<br>
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References
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Jun X, Junjia Y, and Lihong VW. “Photoacoustic Tomography: Principles and Advances.” Progress in Electromagnetics Research 147:1-22, 2014.
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Fang H, Maslov K, and Wang LV. “Photoacoustic Doppler Effect from Flowing Small Light-Absorbing Particles.” Physical Review Letters 99:184501, 2007.
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Yuanyuan J, et al. “Violacein as a Genetically-Controlled, Enzymatically Amplified and Photobleaching-Resistance Chromophore for Optoacoustic Bacterial Imaging.” Nature.com. Nature Publishing Group. 19 June 2015. Web. 18 May 2016.
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E. Chromi. 2009. (18 May 2016; https://2009.igem.org/Team:Cambridge)
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USCF iGEM 2012. 2012. (18 May 2016; https://2012.igem.org/Team:UCSF)
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</p>
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<h2> Welcome to iGEM 2016! </h2>
 
<h2> Welcome to iGEM 2016! </h2>
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<h5> Editing your wiki </h5>
 
<h5> Editing your wiki </h5>
 
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
 
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
  
 
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<h5> Uploading pictures and files </h5>
 
<h5> Uploading pictures and files </h5>
 
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
  
  
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Revision as of 14:33, 1 July 2016

project description

The photoacoustic effect describes the conversion of electromagnetic energy to mechanical energy, namely, that an object absorbing non-ionizing laser pulses experiences local thermal expansions, and vibrates with frequencies in the ultrasonic range which may be detected. Imaging based on this effect yields high contrast from the optical component, and high resolution from the acoustic component (1). For biomedical purposes, users of this technique take advantage of endogenous and exogenous contrast agents to obtain physiological information from the biological tissue, endogenous examples including oxy- and deoxy-hemoglobin to determine blood flow speed (2). The bacterial pigment Violacein (Vio) has been reported to be an effective contrast agent under this technique (3). Furthermore, previous iGEM teams have developed and optimized a biosynthesis pathway for this pigment (4, 5). This team seeks to build upon, and move forward from, these past investigations and develop a biosensor in E. coli to produce Violacein in the presence of significant concentrations of biomarkers for disease; naturally, the team’s search for potential biomarkers will be for those which may pass through an animal’s or human’s gastrointestinal tract. This team considers the additional design aspect of logic gates to modulate the specificity for our system. Alongside Violacein, this team will experiment with similar genetic circuits using the fluorescent protein iRFP. For the purposes of testing the resultant system(s), this team has made an arrangement with a group at MD Anderson who can introduce our bacteria into mice, and who have photoacoustic imaging equipment to image the bacteria within the gastrointestinal tracts of the mice.
References Jun X, Junjia Y, and Lihong VW. “Photoacoustic Tomography: Principles and Advances.” Progress in Electromagnetics Research 147:1-22, 2014. Fang H, Maslov K, and Wang LV. “Photoacoustic Doppler Effect from Flowing Small Light-Absorbing Particles.” Physical Review Letters 99:184501, 2007. Yuanyuan J, et al. “Violacein as a Genetically-Controlled, Enzymatically Amplified and Photobleaching-Resistance Chromophore for Optoacoustic Bacterial Imaging.” Nature.com. Nature Publishing Group. 19 June 2015. Web. 18 May 2016. E. Chromi. 2009. (18 May 2016; https://2009.igem.org/Team:Cambridge) USCF iGEM 2012. 2012. (18 May 2016; https://2012.igem.org/Team:UCSF)