Difference between revisions of "Team:Newcastle/Notebook/Lab/Lightbulb/Week 12"
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Revision as of 17:49, 16 October 2016
September 5th 2016
We planned to mini prep our cell samples but Matt said that the IDT samples had not grown enough so we incubated them for another day. We then designed primers for PCR primer. In the afternoon we mini-prepped the DNA 2.0 using Invitrogen Purelink Quick Plasmid Mini-prep kit.
September 6th 2016
This morning we min-pepped our cells after they had been left to grow for a further 24 hours. This was so we could send our constructs to iGEM headquarters and get them sequenced. Again we used the Invitrogen Purelink Quick Plasmid Mini-prep kit. We did have 9 samples (using 50uL plate samples) but after a slight mishap, we were left with 8 samples.
