Line 27: | Line 27: | ||
<h1 style="color:#66b3ff">Insert title of page here!</h1> | <h1 style="color:#66b3ff">Insert title of page here!</h1> | ||
</div>--> | </div>--> | ||
− | |||
− | |||
<!---------------------------Main content section of page-------------------------------> | <!---------------------------Main content section of page-------------------------------> | ||
Line 36: | Line 34: | ||
<p>We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator. | <p>We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator. | ||
</p> | </p> | ||
− | |||
− | |||
− | |||
− | + | <img style="height:310px; width: 310px" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png"/> | |
<br><i></i></span> | <br><i></i></span> | ||
− | |||
− | + | <span class="ptitle">Bba_K1879000</span><br><br> | |
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence. | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence. | ||
− | + | ||
− | + | ||
<br><br><span class="ptitle">Bba_K1879001</span><br><br> | <br><br><span class="ptitle">Bba_K1879001</span><br><br> | ||
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | ||
</span></p> | </span></p> | ||
− | |||
− | |||
</div> | </div> | ||
− | |||
</div> | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 17:32, 16 October 2016
Overview
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.